Chloride Intracellular Channel 4 (CLIC4) (N-Term) antibody

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Antigen
Epitope
N-Term
(28), (15), (7), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(91), (53), (50), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(80), (20), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(84), (62), (36), (28), (19), (11), (8), (6), (3), (1)
Pubmed 2 references available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the N terminal of human CLIC4
Sequence LSMPLNGLKE EDKEPLIELF VKAGSDGESI GNCPFSQRLF MILWLKGVVF
Predicted Reactivity Human : 100 %, Mouse : 100 %, Rat : 100 %, Bovine : 90 %, Dog : 90 %
Characteristics This is a rabbit polyclonal antibody against CLIon Channel4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name CLIC4 (CLIC4 Antibody Abstract)
Background Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIon Channel4) protein, encoded by the CLIon Channel4 gene, is a member of the p64 family, the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
Molecular Weight 28 kDa
Gene ID 25932
NCBI Accession NP_039234, NM_013943
UniProt Q5VSX5
Research Area Signaling, Metabolism, Cell Cycle, Apoptosis/Necrosis
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 253 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Chloride Intracellular Channel 4 (CLIC4) (N-Term) antibody (ABIN183143) WB Suggested Anti-CLIC4 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:...
Background publications Shiio, Aebersold: "Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry." in: Nature protocols, Vol. 1, Issue 1, pp. 139-45, 2007 (PubMed).

Shiio, Suh, Lee et al.: "Quantitative proteomic analysis of myc-induced apoptosis: a direct role for Myc induction of the mitochondrial chloride ion channel, mtCLIC/CLIC4." in: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2750-6, 2006 (PubMed).

Catalog No. ABIN183143
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