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Splicing Factor Proline/glutamine-Ric (SFPQ) (N-Term) antibody

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Antigen
Epitope
N-Term
(18), (15), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(61), (13), (11), (3), (2), (1), (1), (1), (1)
Host
Rabbit
(44), (14), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(51), (28), (19), (14), (12), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the N terminal of human SFPQ
Sequence VPGPGPGPKQ GPGPGGPKGG KMPGGPKPGG GPGLSTPGGH PKPPHRGGGE
Predicted Reactivity Bovine : 100 %, Human : 100 %, Mouse : 70 %, Rat : 70 %
Characteristics This is a rabbit polyclonal antibody against SFPQ. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name SFPQ (SFPQ Antibody Abstract)
Background SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
Molecular Weight 78 kDa
Gene ID 6421
NCBI Accession NP_005057, NM_005066
UniProt P23246
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 707 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Splicing Factor Proline/glutamine-Ric (SFPQ) (N-Term) antibody (ABIN183984) WB Suggested Anti-SFPQ Antibody Titration: 1.25ug/ml
ELISA Titer: 1:...
Immunohistochemistry (IHC) image for anti-Splicing Factor Proline/glutamine-Ric (SFPQ) (N-Term) antibody (ABIN183984) anti-Splicing Factor Proline/glutamine-Ric (SFPQ) (N-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Splicing Factor Proline/glutamine-Ric (SFPQ) (N-Term) antibody (ABIN183984) anti-Splicing Factor Proline/glutamine-Ric (SFPQ) (N-Term) antibody (Image 3)
Western Blotting (WB) image for anti-Splicing Factor Proline/glutamine-Ric (SFPQ) (N-Term) antibody (ABIN183984) WB Suggested Anti-SFPQ
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:12500
Background publications Rosonina, Ip, Calarco et al.: "Role for PSF in mediating transcriptional activator-dependent stimulation of pre-mRNA processing in vivo." in: Molecular and cellular biology, Vol. 25, Issue 15, pp. 6734-46, 2005 (PubMed).

Catalog No. ABIN183984

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