SFPQ antibody (N-Term)

Catalog No. ABIN2778925

The Rabbit Polyclonal anti-SFPQ antibody (ABIN2778925) specifically detects SFPQ in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow and Dog samples.

Quick Overview for SFPQ antibody (N-Term) (ABIN2778925)

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFPQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SFPQ Antibody

Binding Specificity: N-Term

Purpose: SFPQ Antibody - N-terminal region

Sequence: VPGPGPGPKQ GPGPGGPKGG KMPGGPKPGG GPGLSTPGGH PKPPHRGGGE

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against SFPQ. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SFPQ

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40572-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SFPQ

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Alternative Name: SFPQ

Background:

Background Information: SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

Gene Name: Splicing factor proline/glutamine-rich

Alternative Symbols: PSF, POMP100, PPP1R140

Protein Name: Splicing factor, proline- and glutamine-rich

Molecular Weight: 78kDa

Gene ID: 6421

NCBI Accession: NP_005057

UniProt: P23246