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Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) (C-Term) antibody

Details for Product No. ABIN184570
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Antigen
Synonyms DDB1, xpe, ddba, xap1, xpce, xpe-bf, uv-ddb1, ddb1, 127kDa, AA408517, p127-Ddb1, DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF
Epitope
C-Term
(9), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(55), (29), (27), (14), (12), (8), (2)
Host
Goat
(33), (14), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(45), (17), (14), (10), (10), (8), (6), (4), (1), (1)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN184570
374.27 $
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Immunogen Peptide with sequence C-DLIKVVEELTRIH, from the C Terminus of the protein sequence according to NP_001914.3.
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DDB1
Background Synonyms: DDB1, damage-specific DNA binding protein 1, 127kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD), XPE
Gene ID 1642, 64470
NCBI Accession NP_001914.3
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 130 kDa band seen in HeLa, Jurkat and NSO (mouse) cell lysates. Recommended for use at 0.5-1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) (C-Term) antibody ABIN184570 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.
Product cited in: Li, Robert, van Breugel et al.: "A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery." in: Nature structural & molecular biology, Vol. 17, Issue 1, pp. 105-11, 2010 (PubMed).

General Dualan, Brody, Keeney et al.: "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein." in: Genomics, Vol. 29, Issue 1, pp. 62-9, 1996 (PubMed).

Validation Images
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