GRB2-Related Adaptor Protein (GRAP) (C-Term) antibody

Details for Product No. ABIN184595
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Antigen
Synonyms GRAP, grap, zgc:110202, MGC109892, si:dkeyp-9d4.1, 8430435N19Rik, BB233594
Epitope
C-Term
(3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(35), (21), (19), (1)
Host
Goat
(31), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(24), (12), (10), (6), (5), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184595
374.27 $
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Immunogen Peptide with sequence C-GFFPRSYVQPVHL, from the C Terminus of the protein sequence according to NP_006604.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GRAP
Background Synonyms: GRAP, GRB2-related adaptor protein, growth factor receptor-bound protein 2-related adaptor protein, MGC64880
Gene ID 10750, 71520
NCBI Accession NP_006604
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26 kDa band observed in human thymus lysates (calculated MW of 25.3 kDa according to NP_006604.1). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-GRB2-Related Adaptor Protein (GRAP) (C-Term) antibody ABIN184595 (0.3µg/ml) staining of human thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Feng, Ouyang, Hu et al.: "Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway." in: The Journal of biological chemistry, Vol. 271, Issue 21, pp. 12129-32, 1996 (PubMed).

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