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Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibody

Details for Product No. ABIN184638, Supplier: Log in to see
Antigen
  • DUSP1
  • cb269
  • sb:cb269
  • zgc:55920
  • id:ibd2586
  • wu:fd19g02
  • wu:fe50g09
  • CL100
  • HVH1
  • MKP-1
  • MKP1
  • PTPN10
  • 3CH134
  • Ptpn16
  • U19515
  • erp
  • mkp-1
  • Mkp1
Epitope
C-Term
19
18
18
13
5
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
106
42
30
6
4
3
2
2
1
1
1
1
Host
Goat
82
18
6
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
73
51
32
25
16
7
2
1
1
Supplier
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Immunogen Peptide with sequence SYLQSPITTSPSC, from the C Terminus of the protein sequence according to NP_004408.1.
Blocking Peptide Blocking peptide for this product available: ABIN370353
Cross-Reactivity Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dual specificity protein phosphatase 1 (DUSP1/MKP-1) (DUSP1 Antibody Abstract)
Background Synonyms: DUSP1, dual specificity phosphatase 1, HVH1, CL100, MKP-1, PTPN10, serine/threonine specific protein phosphatase, MKP1
Gene ID 1843, 19252
NCBI Accession NP_004408
Research Area Signaling
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 40 kDa band observed in Human lysates of HeLa (calculated MW of 39.3 kDa according to NP_004408.1). Recommended concentration: 0.3-1 µg/mL. An anonymous customer found positive results in WB on Mouse microglia (BV2 cells). An anonymous customer found positive results in WB on Rat primary microglia. An anonymous customer found positive results in WB on Rat spinal cord homogenate.
IHC: In paraffin embedded Human Prostate shows strong cytoplasm staining of secretory cells in the glands. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibody (ABIN184638) ABIN184638 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen r...
 image for anti-Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibody (ABIN184638) ABIN184638 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary ...
Background publications Alessi, Smythe, Keyse: "The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts." in: Oncogene, Vol. 8, Issue 7, pp. 2015-20, 1993 (PubMed).