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Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibody

Details for Product No. ABIN184638
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Antigen
Synonyms DUSP1, cb269, sb:cb269, zgc:55920, id:ibd2586, wu:fd19g02, wu:fe50g09, CL100, HVH1, MKP-1, MKP1, PTPN10, 3CH134, Ptpn16, U19515, erp, mkp-1, Mkp1
Epitope
C-Term
(25), (18), (13), (8), (5), (4), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(95), (54), (38), (38), (36), (30), (24)
Host
Goat
(83), (8), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(50), (40), (32), (21), (15), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184638
374.27 $
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Immunogen Peptide with sequence SYLQSPITTSPSC, from the C Terminus of the protein sequence according to NP_004408.1.
Cross-Reactivity Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dual specificity protein phosphatase 1 (DUSP1/MKP-1)
Background Synonyms: DUSP1, dual specificity phosphatase 1, HVH1, CL100, MKP-1, PTPN10, serine/threonine specific protein phosphatase, MKP1
Gene ID 1843, 19252
NCBI Accession NP_004408.1
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 40 kDa band observed in Human lysates of HeLa (calculated MW of 39.3 kDa according to NP_004408.1). Recommended concentration: 0.3-1 µg/mL. An anonymous customer found positive results in WB on Mouse microglia (BV2 cells). An anonymous customer found positive results in WB on Rat primary microglia. An anonymous customer found positive results in WB on Rat spinal cord homogenate.
IHC: In paraffin embedded Human Prostate shows strong cytoplasm staining of secretory cells in the glands. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibody ABIN184638 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-Dual Specificity Phosphatase 1 (DUSP1) (C-Term) antibody (2) ABIN184638 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Alessi, Smythe, Keyse: "The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts." in: Oncogene, Vol. 8, Issue 7, pp. 2015-20, 1993 (PubMed).

Validation Images
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