FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibody

Details for Product No. ABIN184712
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Synonyms FXYD5, DYSAD, IWU1, KCT1, OIT2, PRO6241, RIC, EF-8, Oit2
(18), (15), (15), (5), (3), (1), (1)
(51), (1), (1), (1)
(32), (19)
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(42), (23), (19), (6), (3), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184712
325.97 $
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Immunogen Peptide with sequence GKCRQLSRLCRNHCR, from the C Terminus of the protein sequence according to NP_054883.3, NP_659003.1, NP_001158077.1.
Specificity NP_054883.3, NP_659003.1and NP_001158077.1 are variants that represent the same protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dysadherin
Background Synonyms: FXYD5, dysadherin, FXYD domain containing ion transport regulator 5, RIC, IWU1, IWU-1, dysad, HSPC113, KCT1, OIT2, PRO6241, FXYD domain-containing ion transport regulator 5, keratinocytes associated transmembrane protein 1, DYSAD, HSPC113
Gene ID 53827
NCBI Accession NP_659003, NP_001158077, NP_054883
Research Area Signaling, Cytoskeleton, Microfilaments
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 38 kDa band in Human Spleen lysate and an approx 26 kDa in Human Lung lysate after 1 µg/mL antibody staining. The bands were successfully blocked by incubation with the immunizing peptide. Please note that according to the literature this protein is O-glycosylated (PMID: 12672699), which may explain the different sizes of bands we observe given the calculated size of 19.5 kDa according to NP_054883 and NP_659003.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibody ABIN184712 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Ino, Gotoh, Sakamoto et al.: "Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 1, pp. 365-70, 2002 (PubMed).

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