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FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibody

Details for Product No. ABIN184712
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Antigen
Synonyms DYSAD, IWU1, KCT1, OIT2, PRO6241, RIC, EF-8, Oit2, FXYD5
Epitope
C-Term
(8), (8), (3), (3), (2)
Reactivity
Human
(23)
Host
Goat
(20), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(24), (10), (8), (3)
Pubmed 1 reference available
Quantity 100 µg
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Availability Available
Catalog No. ABIN184712
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence GKCRQLSRLCRNHCR, from the C Terminus of the protein sequence according to NP_054883.3, NP_659003.1, NP_001158077.1.
Specificity NP_054883.3, NP_659003.1and NP_001158077.1 are variants that represent the same protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dysadherin
Background Synonyms: FXYD5, dysadherin, FXYD domain containing ion transport regulator 5, RIC, IWU1, IWU-1, dysad, HSPC113, KCT1, OIT2, PRO6241, FXYD domain-containing ion transport regulator 5, keratinocytes associated transmembrane protein 1, DYSAD, HSPC113
Gene ID 53827
NCBI Accession NP_054883.3, NP_659003.1, NP_001158077.1
Research Area Signaling, Cytoskeleton, Microfilaments
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 38 kDa band in Human Spleen lysate and an approx 26 kDa in Human Lung lysate after 1 µg/mL antibody staining. The bands were successfully blocked by incubation with the immunizing peptide. Please note that according to the literature this protein is O-glycosylated (PMID: 12672699), which may explain the different sizes of bands we observe given the calculated size of 19.5 kDa according to NP_054883 and NP_659003.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibody ABIN184712 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Ino, Gotoh, Sakamoto et al.: "Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 1, pp. 365-70, 2002 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN184712
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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