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ARPC2 antibody (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa) (C-Term)

Details for Product anti-ARPC2 Antibody No. ABIN184748, Supplier: Login to see
Antigen
  • arc34
  • p34-arc
  • pnas-139
  • pro2446
  • Arpc2
  • Arc-p34
  • 2210023N03Rik
  • 34kDa
  • p34-Arc
  • fk84a07
  • wu:fa22f07
  • wu:fk84a07
  • zgc:77769
  • ARC34
  • PNAS-139
Alternatives
anti-Human ARPC2 antibody for ELISA
Epitope
C-Term
34
17
13
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
97
34
33
7
5
2
1
1
1
1
Host
Goat
73
20
6
Clonality
Polyclonal
Conjugate
This ARPC2 antibody is un-conjugated
8
8
8
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
78
54
31
16
12
11
6
3
1
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1.
Blocking Peptide Blocking peptide for this product available: ABIN370297
Specificity The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ARPC2 (ARPC2 Antibody Abstract)
Background Synonyms: ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2
Gene ID 10109, 76709
NCBI Accession NP_005722, NP_690601
Research Area Signaling, Cytoskeleton, Microfilaments
Pathways RTK Signaling
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 33-34 kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34 kDa according to NP_005722). Recommended for use at 0.1-0.5 µg/mL.
IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Sakata, Taniguchi, Yasuda et al.: "Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1." in: The Journal of experimental medicine, Vol. 204, Issue 9, pp. 2031-8, 2007 (PubMed).

Background publications Welch, DePace, Verma et al.: "The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly." in: The Journal of cell biology, Vol. 138, Issue 2, pp. 375-84, 1997 (PubMed).