Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (C-Term) antibody

Details for Product No. ABIN184748
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Antigen
Synonyms arc34, p34-arc, pnas-139, pro2446, Arpc2, Arc-p34, 2210023N03Rik, 34kDa, p34-Arc, fk84a07, wu:fa22f07, wu:fk84a07, zgc:77769, ARC34, PNAS-139
Epitope
C-Term
(12), (7), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(46), (24), (23), (17), (15), (14), (1), (1), (1)
Host
Goat
(37), (9), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(38), (22), (14), (10), (8), (6), (4)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN184748
374.27 $
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Immunogen Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1.
Specificity The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ARPC2
Background Synonyms: ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2
Gene ID 10109, 76709
NCBI Accession NP_005722, NP_690601
Research Area Signaling, Cytoskeleton, Microfilaments
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 33-34 kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34 kDa according to NP_005722). Recommended for use at 0.1-0.5 µg/mL.
IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Sakata, Taniguchi, Yasuda et al.: "Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1." in: The Journal of experimental medicine, Vol. 204, Issue 9, pp. 2031-8, 2007 (PubMed).

General Welch, DePace, Verma et al.: "The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly." in: The Journal of cell biology, Vol. 138, Issue 2, pp. 375-84, 1997 (PubMed).

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