Kinesin Family Member 5B (KIF5B) (C-Term) antibody

Details for Product No. ABIN184774
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Antigen
Synonyms Khc, KIF5A, KINH, KNS, KNS1, UKHC, AL022807, Khcs, Kns1, Ukhc, khc
Epitope
C-Term
(13), (7), (4), (3), (3), (1), (1), (1)
Reactivity
Human, Dog (Canine)
(40), (14), (14), (11), (11), (9), (2), (2), (2), (2)
Host
Goat
(25), (24), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(44), (26), (18), (17), (12), (12), (9), (4), (1)
Pubmed 3 references available
Quantity 100 µg
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Catalog No. ABIN184774
374.27 $
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Immunogen Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Kinesin 1 / UKHC
Background Synonyms: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
Gene ID 3799
NCBI Accession NP_004512
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration: 1-2 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat). In paraffin embedded Human Uterus shows strong staining of the epithelium on endometrial glands. Recommended concentration: 2-3 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody ABIN184774 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody (2) ABIN184774 (1µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Product cited in: Gupta, Palmer, Spence et al.: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, 2008 (PubMed).

Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, 2010 (PubMed).

General Semiz, Park, Nicoloro et al.: "Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules." in: The EMBO journal, Vol. 22, Issue 10, pp. 2387-99, 2003 (PubMed).

Validation Images
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