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Details for Product No. ABIN184774

Kinesin Family Member 5B (KIF5B) (C-Term) antibody

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Antigen
Synonyms KNS, KINH, KNS1, UKHC, AL022807, Khc, Khcs, Kns1, Ukhc, khc, KIF5A
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Dog (Canine)
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Catalog no. ABIN184774
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Kinesin 1 / UKHC
Background Synonyms: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
Gene ID 3799
NCBI Accession NP_004512.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration: 1-2 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat). In paraffin embedded Human Uterus shows strong staining of the epithelium on endometrial glands. Recommended concentration: 2-3 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Gupta, Palmer, Spence et al.: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, 2008 (PubMed).

Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, 2010 (PubMed).

Semiz, Park, Nicoloro et al.: "Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules." in: The EMBO journal, Vol. 22, Issue 10, pp. 2387-99, 2003 (PubMed).

Alternatives for antigen "Kinesin Family Member 5B (KIF5B)", type "Antibodies"
Hosts (28), (19), (10)
Reactivities (35), (18), (16), (12), (12), (9), (2), (2), (2), (2)
Applications (39), (21), (18), (16), (15), (10), (5), (2), (2), (1), (1)
Epitopes (9), (4), (3), (2), (2)
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