Kinesin Family Member 5B (KIF5B) (C-Term) antibody

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Antigen
Synonyms Khc, KIF5A, KINH, KNS, KNS1, UKHC, AL022807, Khcs, Kns1, Ukhc, khc
Epitope
C-Term
(35), (16), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Dog (Canine)
(65), (18), (16), (14), (13), (8), (2), (2), (2), (2), (1), (1), (1)
Host
Goat
(38), (27), (19)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(67), (43), (27), (19), (15), (9), (9), (6), (5), (3), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 100 μg
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Immunogen Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Blocking Peptide Blocking peptide for this product available: Kinesin Family Member 5B (KIF5B) peptide (ABIN369683)
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Kinesin 1 / UKHC (KIF5B Antibody Abstract)
Background Synonyms: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
Gene ID 3799
NCBI Accession NP_004512
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration: 1-2 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat). In paraffin embedded Human Uterus shows strong staining of the epithelium on endometrial glands. Recommended concentration: 2-3 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody (ABIN184774) ABIN184774 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protei...
Immunohistochemistry (IHC) image for anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody (ABIN184774) ABIN184774 (1µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen re...
Product cited in: Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, 2010 (PubMed).

Gupta, Palmer, Spence et al.: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, 2008 (PubMed).

Background publications Semiz, Park, Nicoloro et al.: "Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules." in: The EMBO journal, Vol. 22, Issue 10, pp. 2387-99, 2003 (PubMed).

Catalog No. ABIN184774
$221.76
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