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Kinesin Family Member 5B (KIF5B) (C-Term) antibody

Details for Product No. ABIN184774, Supplier: Login to see
Antigen
  • Khc
  • KIF5A
  • KINH
  • KNS
  • KNS1
  • UKHC
  • AL022807
  • Khcs
  • Kns1
  • Ukhc
  • khc
Epitope
C-Term
35
16
4
3
3
1
1
1
1
1
1
1
Reactivity
Human, Dog (Canine)
66
18
17
15
13
8
2
2
2
2
1
1
1
Host
Goat
39
27
19
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
68
43
28
19
15
10
9
6
5
3
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Blocking Peptide Blocking peptide for this product available: ABIN369683
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Kinesin 1 / UKHC (KIF5B Antibody Abstract)
Background Synonyms: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
Gene ID 3799
NCBI Accession NP_004512
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration: 1-2 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat). In paraffin embedded Human Uterus shows strong staining of the epithelium on endometrial glands. Recommended concentration: 2-3 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody (ABIN184774) ABIN184774 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protei...
Immunohistochemistry (IHC) image for anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody (ABIN184774) ABIN184774 (1µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen re...
Product cited in: Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, 2010 (PubMed).

Gupta, Palmer, Spence et al.: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, 2008 (PubMed).

Background publications Semiz, Park, Nicoloro et al.: "Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules." in: The EMBO journal, Vol. 22, Issue 10, pp. 2387-99, 2003 (PubMed).