Kinesin Family Member 5B (KIF5B) (C-Term) antibody

Details for Product No. ABIN184774
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms KNS, KINH, KNS1, UKHC, AL022807, Khc, Khcs, Kns1, Ukhc, khc, KIF5A
(7), (4), (3), (2), (1), (1)
Human, Dog (Canine)
(30), (12), (12), (12), (11), (8), (2), (2), (2), (2)
(26), (17), (9)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(38), (21), (18), (17), (12), (11), (7), (4), (2), (1)
Pubmed 3 references available
Catalog no. ABIN184774
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Available

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Kinesin 1 / UKHC
Background Synonyms: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
Gene ID 3799
NCBI Accession NP_004512.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration: 1-2 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat). In paraffin embedded Human Uterus shows strong staining of the epithelium on endometrial glands. Recommended concentration: 2-3 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody ABIN184774 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody (2) ABIN184774 (1µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Product cited in: Gupta, Palmer, Spence et al.: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, 2008 (PubMed).

Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, 2010 (PubMed).

General Semiz, Park, Nicoloro et al.: "Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules." in: The EMBO journal, Vol. 22, Issue 10, pp. 2387-99, 2003 (PubMed).

Hosts (26), (17), (9)
Reactivities (30), (12), (12), (12), (11), (8), (2), (2), (2), (2)
Applications (38), (21), (18), (17), (12), (11), (7), (4), (2), (1)
Epitopes (7), (4), (3), (2), (1), (1)
back to top