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Interferon Regulatory Factor 2 (IRF2) (C-Term) antibody

Details for Product No. ABIN184810, Supplier: Login to see
Antigen
  • IRF-2
  • 9830146E22Rik
  • AI646973
  • Irf-2
  • irf2b
  • zgc:103451
  • irf-2
Epitope
C-Term
21
17
16
14
10
5
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
116
51
36
7
5
2
2
1
1
1
1
1
Host
Goat
87
18
17
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
97
52
19
12
11
8
6
3
2
1
1
1
Supplier
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Immunogen Peptide with sequence KTSDITQARVKSC, from the C Terminus of the protein sequence according to NP_002190.2.
Blocking Peptide Blocking peptide for this product available: ABIN370232
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name IRF2 (IRF2 Antibody Abstract)
Background Synonyms: IRF2, interferon regulatory factor 2, DKFZp686F0244, IRF-2
Gene ID 3660, 16363
NCBI Accession NP_002190
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Innate Immunity, Cytokines
Pathways
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 48 kDa band observed in lysates of cell line Jurkat (calculated MW of 39.3 kDa according to NP_002190.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr,40(5):976-82., PMID: 10102295. In transfected HEK293 transiently expressing IRF2 bands of approx. 40 kDa and 45 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Interferon Regulatory Factor 2 (IRF2) (C-Term) antibody (ABIN184810) ABIN184810 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per la...
Product cited in: Liljeroos, Vuolteenaho, Rounioja et al.: "Bacterial ligand of TLR2 signals Stat activation via induction of IRF1/2 and interferon-alpha production." in: Cellular signalling, Vol. 20, Issue 10, pp. 1873-81, 2008 (PubMed).

Background publications Harada, Kitagawa, Tanaka et al.: "Anti-oncogenic and oncogenic potentials of interferon regulatory factors-1 and -2." in: Science (New York, N.Y.), Vol. 259, Issue 5097, pp. 971-4, 1993 (PubMed).