Interferon Regulatory Factor 5 (IRF5) (C-Term) antibody

Details for Product No. ABIN184812
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Antigen
Synonyms IRF5, irf5, SLEB10, AW491843, mirf5, im:7155364, zgc:76986
Epitope
C-Term
(8), (8), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(56), (23), (12), (5), (4), (2), (1), (1), (1)
Host
Goat
(30), (28), (8), (1)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(59), (32), (20), (12), (11), (9), (7), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN184812
374.27 $
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Immunogen Peptide with sequence C-QGPWPMHPAGMQ, from the C Terminus of the protein sequence according to NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1.
Specificity This antibody is expected to recognize all four reported isoforms (NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein:
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name IRF5
Background Synonyms: IRF5, interferon regulatory factor 5, SLEB10
Gene ID 3663
NCBI Accession NP_116032, NP_001092099, NP_001092097, NP_002191
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Innate Immunity, Cytokines
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 57 kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8 kDa according to NP_001092099.1. Recommended concentration: 0.1-0.3 µg/mL. Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Interferon Regulatory Factor 5 (IRF5) (C-Term) antibody ABIN184812 (0.1µg/ml) staining of lysate of cell line A549 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Interferon Regulatory Factor 5 (IRF5) (C-Term) antibody (2) HEK293 overexpressing human IRF5 and probed with ABIN184812 at 1ug/ml (mock transfection in lane B).
General Barnes, Moore, Pitha: "Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23382-90, 2001 (PubMed).

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