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Centromere Protein F (CENPF) antibody

Details for Product No. ABIN184827
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Antigen
Synonyms CENF, hcp-1, PRO1779, CMF1, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, Lek1, mitosin, AI325968, 6530404A22Rik, LOC100222985
Reactivity
Human
(41), (1), (1), (1)
Host
Goat
(27), (11), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA
(20), (16), (16), (10), (9), (7), (6), (3), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Availability Available
Catalog No. ABIN184827
261.61 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen GLESKGSENCKVQ
Specificity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Centromere protein F
Background All names and symbols: CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein. Official Symbol: CENPF. RefSeq number(s): NP_057427. Gene ontology terms: cytoplasm, glutathione transferase activity, glutathione transferase activity, metabolism, transferase activity
Gene ID 1063
Application Notes Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
General Liao, Winkfein, Mack et al.: "CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis." in: The Journal of cell biology, Vol. 130, Issue 3, pp. 507-18, 1995 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN184827
261.61 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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