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CENPF antibody (Centromere Protein F)

Details for Product anti-CENPF Antibody No. ABIN184827, Supplier: Login to see
Antigen
  • LOC100222985
  • 6530404A22Rik
  • AI325968
  • CENF
  • Lek1
  • PRO1779
  • mitosin
  • im:7140452
  • im:7144238
  • im:7154719
  • im:7156761
  • si:ch211-152h14.2
  • hcp-1
  • CMF1
Reactivity
Human
56
3
3
3
1
Host
Goat
21
18
18
Clonality
Polyclonal
Conjugate
This CENPF antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
Application
ELISA
24
20
19
18
14
14
6
5
3
2
1
1
Supplier
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Supplier Product No.
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Immunogen GLESKGSENCKVQ
Blocking Peptide Blocking peptide for this product available: ABIN370157
Specificity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Centromere protein F (CENPF Antibody Abstract)
Background All names and symbols: CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein. Official Symbol: CENPF. RefSeq number(s): NP_057427. Gene ontology terms: cytoplasm, glutathione transferase activity, glutathione transferase activity, metabolism, transferase activity
Gene ID 1063
Pathways
Application Notes Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Liao, Winkfein, Mack et al.: "CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis." in: The Journal of cell biology, Vol. 130, Issue 3, pp. 507-18, 1995 (PubMed).