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Details for Product No. ABIN184827

Centromere Protein F (CENPF) antibody

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Antigen
Synonyms CENF, hcp-1, PRO1779, CMF1, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, Lek1, mitosin, AI325968, 6530404A22Rik, LOC100222985
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA
Pubmed 1 reference available
Catalog no. ABIN184827
Quantity 100 µg
Price
261.61 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen GLESKGSENCKVQ
Specificity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Centromere protein F
Background All names and symbols: CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein. Official Symbol: CENPF. RefSeq number(s): NP_057427. Gene ontology terms: cytoplasm, glutathione transferase activity, glutathione transferase activity, metabolism, transferase activity
Gene ID 1063
Application Notes Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Storage -20 °C
Liao, Winkfein, Mack et al.: "CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis." in: The Journal of cell biology, Vol. 130, Issue 3, pp. 507-18, 1995 (PubMed).

Alternatives for antigen "Centromere Protein F (CENPF)", type "Antibodies"
Hosts (29), (12), (6)
Reactivities (44), (1), (1), (1), (1), (1)
Applications (27), (19), (16), (11), (9), (7), (7), (2), (1), (1)
Conjugates (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (4)
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