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Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibody

Details for Product No. ABIN184891, Supplier: Login to see
Antigen
  • e2-25k
  • hip2
  • hypg
  • lig
  • HIP2
  • E2-25K
  • HYPG
  • LIG
  • UBC1
  • AW492011
  • D5Ertd601e
  • E2-25k
  • Hip2
  • Hypg
  • Lig
  • ube2k
  • zgc:103472
Epitope
C-Term
24
18
15
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
110
32
22
12
11
10
6
6
6
4
Host
Goat
81
19
9
2
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
3
3
3
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
93
53
28
9
4
3
3
3
2
1
1
Supplier
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Immunogen Peptide with sequence C-SWDVETATELLLSN, from the C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1.
Blocking Peptide Blocking peptide for this product available: ABIN369945
Specificity This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
Cross-Reactivity Mouse (Murine), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name HIP2 (UBE2K Antibody Abstract)
Background Synonyms: UBE2K, HIP2, huntingtin interacting protein 2, LIG, HYPG, ubiquitin-protein ligase, ubiquitin carrier protein, ubiquitin-conjugating enzyme E2-25 KDA, ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), DKFZp564C1216, DKFZp686J24237, E2-25K
Gene ID 3093
NCBI Accession NP_005330, NP_001104582, NP_001104583
Research Area Proteolysis / Ubiquitin
Pathways
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 24 kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibody (ABIN184891) ABIN184891 staining (1.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per ...
Background publications Kalchman, Graham, Xia et al.: "Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme." in: The Journal of biological chemistry, Vol. 271, Issue 32, pp. 19385-94, 1996 (PubMed).