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Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibody

Details for Product No. ABIN184891
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Antigen
Synonyms e2-25k, hip2, hypg, lig, HIP2, E2-25K, HYPG, LIG, UBC1, AW492011, D5Ertd601e, E2-25k, Hip2, Hypg, Lig, ube2k, zgc:103472
Epitope
C-Term
(14), (7), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(61), (29), (19), (18), (18), (16), (12), (5), (4)
Host
Goat
(50), (6), (6), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(51), (17), (10), (9), (8), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN184891
374.27 $
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Immunogen Peptide with sequence C-SWDVETATELLLSN, from the C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1.
Specificity This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
Cross-Reactivity Mouse (Murine), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name HIP2
Background Synonyms: UBE2K, HIP2, huntingtin interacting protein 2, LIG, HYPG, ubiquitin-protein ligase, ubiquitin carrier protein, ubiquitin-conjugating enzyme E2-25 KDA, ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), DKFZp564C1216, DKFZp686J24237, E2-25K
Gene ID 3093
NCBI Accession NP_005330.1, NP_001104582.1, NP_001104583.1
Research Area Proteolysis / Ubiquitin
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 24 kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibody ABIN184891 staining (1.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Kalchman, Graham, Xia et al.: "Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme." in: The Journal of biological chemistry, Vol. 271, Issue 32, pp. 19385-94, 1996 (PubMed).

Validation Images
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