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Tripartite Motif Containing 24 (TRIM24) (C-Term) antibody

Details for Product No. ABIN184911, Supplier: Login to see
Antigen
  • TRIM24
  • PTC6
  • RNF82
  • TF1A
  • TIF1
  • TIF1A
  • TIF1ALPHA
  • hTIF1
  • ptc6
  • rnf82
  • tf1a
  • tif1
  • tif1a
  • tif1alpha
  • A130082H20Rik
  • AI447469
  • D430004I05Rik
  • TIF1-alpha
  • TIF1alpha
  • Tif1a
Epitope
C-Term
27
16
15
14
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
98
25
9
5
3
2
2
1
1
1
1
Host
Goat
65
19
15
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
77
46
23
14
11
8
2
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-KRLKSIEERQLLK, from the C Terminus of the protein sequence according to NP_056989.2, NP_003843.3.
Blocking Peptide Blocking peptide for this product available: ABIN369359
Specificity This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.
Cross-Reactivity Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name TIF1A / TRIM24 (TRIM24 Antibody Abstract)
Background Synonyms: TRIM24, TIF1, transcriptional intermediary factor 1, PTC6, TF1A, TIF1A, Tif1a, hTIF1, TIF1ALPHA, transcriptional intermediary factor 1 alpha, tripartite motif protein 24, tripartite motif-containing 24, RNF82
Gene ID 8805
NCBI Accession NP_056989, NP_003843
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 120 kDa band in human kidney and heart lysates at 1 µg/mL, however prominent bands were also seen at 25 kDa and 45 kDa. Please note that currently we cannot find an explanation in the literature for some of the bands we observe given the predicted sizes of approx. 117 kDa and 121 kDa according to NP_003843 and NP_056989.
IHC: In paraffin embedded Human Breast cancer shows nuclear staining in the distorted lobules. Recommended concentration: 4-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Thénot, Henriquet, Rochefort et al.: "Differential interaction of nuclear receptors with the putative human transcriptional coactivator hTIF1." in: The Journal of biological chemistry, Vol. 272, Issue 18, pp. 12062-8, 1997 (PubMed).