Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) (C-Term) antibody

Details for Product No. ABIN185061
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Antigen
Synonyms Rabenosyn-5, 5330426D11Rik, zfyve20
Epitope
C-Term
(3), (2), (2)
Reactivity
Human
(15), (9), (1), (1), (1), (1)
Host
Goat
(12), (4)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(15), (9)
Pubmed 1 reference available
Catalog no. ABIN185061
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-ELKHTLAKQKGGTD, from the C Terminus of the protein sequence according to NP_071735.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Rabenosyn 5
Background Synonyms: ZFYVE20, Rabenosyn 5, FYVE-finger-containing Rab5 effector protein rabenosyn-5, zinc finger, FYVE domain containing 20, zinc finger, FYVE domain containing 20, FLJ34993, MGC126210, Rabenosyn-5
Gene ID 64145
NCBI Accession NP_071735.2
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration: 0.3 - 1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) (C-Term) antibody ABIN185061 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Nielsen, Christoforidis, Uttenweiler-Joseph et al.: "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain." in: The Journal of cell biology, Vol. 151, Issue 3, pp. 601-12, 2000 (PubMed).

Hosts (12), (4)
Reactivities (15), (9), (1), (1), (1), (1)
Applications (15), (9)
Epitopes (3), (2), (2)
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