SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1) (C-Term) antibody

Details for Product No. ABIN185062
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Synonyms BAF57, XBaf57, MGC52696, MGC69429, MGC81726, SMARCE1, DKFZp459O2235, fb54d11, wu:fb33d02, wu:fb54d11, Baf57, 2810417B20Rik, 5830412H02Rik, 9030408N19Rik, Baf27, RGD1304726
(35), (14), (4), (4), (2), (2), (1), (1), (1), (1)
(65), (10), (7), (3), (2), (2), (2), (1), (1), (1), (1)
(40), (19), (7)
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(64), (38), (31), (10), (9), (9), (3), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185062
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Immunogen Peptide with sequence C-PPTDPIPEDEKKE, from the C Terminus of the protein sequence according to NP_003070.3.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BAF57 / SMARCE1
Background Synonyms: SMARCE1, BAF57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, mammalian chromatin remodeling complex BRG1-associated factor 57, BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
Gene ID 6605, 57376
NCBI Accession NP_003070
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50 kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6 kDa according to NP_003070.3). A 48 kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 µg/mL. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane), tested by Origene. B) EB06054 staining (0.2 µg/mL) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IHC: In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Wang, Chi, Xue et al.: "Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 492-8, 1998 (PubMed).

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