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SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1) (C-Term) antibody

Details for Product No. ABIN185062
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Antigen
Synonyms BAF57, XBaf57, MGC52696, MGC69429, MGC81726, SMARCE1, DKFZp459O2235, fb54d11, wu:fb33d02, wu:fb54d11, Baf57, Baf27, 2810417B20Rik, 5830412H02Rik, 9030408N19Rik, RGD1304726
Epitope
C-Term
(15), (7), (4), (2), (2), (2)
Reactivity
Human
(37), (10), (6), (2), (2), (2), (2), (2), (1)
Host
Goat
(28), (7), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(37), (16), (16), (8), (5), (3), (2)
Pubmed 1 reference available
Quantity 100 µg
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Availability Available
Catalog No. ABIN185062
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-PPTDPIPEDEKKE, from the C Terminus of the protein sequence according to NP_003070.3.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BAF57 / SMARCE1
Background Synonyms: SMARCE1, BAF57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, mammalian chromatin remodeling complex BRG1-associated factor 57, BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
Gene ID 6605, 57376
NCBI Accession NP_003070.3
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50 kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6 kDa according to NP_003070.3). A 48 kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 µg/mL. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane), tested by Origene. B) EB06054 staining (0.2 µg/mL) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IHC: In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Wang, Chi, Xue et al.: "Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 492-8, 1998 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185062
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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