Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A) (C-Term) antibody

Details for Product No. ABIN185152
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Antigen
Synonyms Par6a, Par-6a, Par6, Par-6, Par6c, TAX40, Tip-40, PAR6alpha, 0710008C04Rik, 2610010A15Rik, PAR6, PAR6C, PAR-6A, TIP-40, PARD6A
Epitope
C-Term
(15), (7), (3), (3), (1), (1)
Reactivity
Human
(41), (17), (13), (12), (10), (1)
Host
Goat
(46), (6), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(37), (23), (10), (9), (7), (4), (2), (1)
Pubmed 1 reference available
Catalog no. ABIN185152
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
Options
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Availability Available

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1, NP_001032358.1.
Specificity This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PAR6alpha / PARD6A
Background Synonyms: PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning defective 6 homolog alpha, partitioning defective-6 homolog alpha, partitioning-defective protein 6
Gene ID 50855
NCBI Accession NP_058644.1, NP_001032358.1
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1 µg/mL.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10 µg/mL. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A) (C-Term) antibody ABIN185152 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
anti-Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A) (C-Term) antibody (2) ABIN185152 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Johansson, Driessens, Aspenström: "The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1." in: Journal of cell science, Vol. 113 ( Pt 18), pp. 3267-75, 2000 (PubMed).

Hosts (46), (6), (3)
Reactivities (41), (17), (13), (12), (10), (1)
Applications (37), (23), (10), (9), (7), (4), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (7), (3), (3), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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