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SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody

Details for Product No. ABIN185183, Supplier: Login to see
Antigen
  • SH3BP1
  • ARHGAP43
  • 3BP-1
Epitope
C-Term
19
15
10
7
3
2
2
1
Reactivity
Human
55
5
1
1
1
Host
Goat
36
19
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
51
32
15
10
9
4
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
Blocking Peptide Blocking peptide for this product available: ABIN369576
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SH3BP1 (SH3BP1 Antibody Abstract)
Background Synonyms: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
Gene ID 23616
NCBI Accession NP_061830
Research Area Signaling
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 µg/mL. Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody (ABIN185183) ABIN185183 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein p...
Immunohistochemistry (IHC) image for anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody (ABIN185183) ABIN185183 (0.3µg/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic stainin...
Product cited in: Parrini, Sadou-Dubourgnoux, Aoki et al.: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, 2011 (PubMed).

Background publications Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22. ..." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).