SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody

Details for Product No. ABIN185183
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Antigen
Synonyms 3BP-1, ARHGAP43, SH3BP1
Epitope
C-Term
(5), (2), (2), (1)
Reactivity
Human
(17), (3)
Host
Goat
(11), (7)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(17), (6), (6), (4), (2)
Pubmed 2 references available
Catalog no. ABIN185183
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SH3BP1
Background Synonyms: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
Gene ID 23616
NCBI Accession NP_061830.3
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 µg/mL. Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody ABIN185183 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody (2) ABIN185183 (0.3µg/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Product cited in: Parrini, Sadou-Dubourgnoux, Aoki et al.: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, 2011 (PubMed).

General Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22...." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Hosts (11), (7)
Reactivities (17), (3)
Applications (17), (6), (6), (4), (2)
Epitopes (5), (2), (2), (1)
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