SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody

Details for Product No. ABIN185183
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Antigen
Synonyms SH3BP1, ARHGAP43, 3BP-1
Epitope
C-Term
(7), (5), (3), (2), (1)
Reactivity
Human
(24), (3)
Host
Goat
(17), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(23), (13), (8), (5), (3), (1)
Pubmed 2 references available
Quantity 100 µg
Options
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Catalog No. ABIN185183
374.27 $
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Immunogen Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SH3BP1
Background Synonyms: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
Gene ID 23616
NCBI Accession NP_061830
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 µg/mL. Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody ABIN185183 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody (2) ABIN185183 (0.3µg/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Product cited in: Parrini, Sadou-Dubourgnoux, Aoki et al.: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, 2011 (PubMed).

General Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22. ..." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

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