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Details for Product No. ABIN185183

SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody

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Antigen
Synonyms 3BP-1, ARHGAP43, SH3BP1
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN185183
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SH3BP1
Background Synonyms: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
Gene ID 23616
NCBI Accession NP_061830.3
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 µg/mL. Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Parrini, Sadou-Dubourgnoux, Aoki et al.: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, 2011 (PubMed).

Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Alternatives for antigen "SH3-Domain Binding Protein 1 (SH3BP1)", type "Antibodies"
Hosts (11), (8)
Reactivities (18), (2)
Applications (18), (7), (7), (3), (2)
Epitopes (5), (2), (2), (1), (1), (1), (1)
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