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SH3BP1 antibody (C-Term)

Verified SH3BP1 Reactivity: Human WB, ELISA, IF, IHC, ICC Host: Goat Polyclonal unconjugated
Catalog No. ABIN185183
  • Target See all SH3BP1 Antibodies
    SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))
    Binding Specificity
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 24
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 24
    Goat
    Clonality
    • 24
    Polyclonal
    Conjugate
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    This SH3BP1 antibody is un-conjugated
    Application
    • 20
    • 17
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Purpose
    SH3BP1
    Sequence
    PQPRPRSLAS ETN
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 μg/mL.

    Western Blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Parrini, Sadou-Dubourgnoux, Aoki, Kunida, Biondini, Hatzoglou, Poullet, Formstecher, Yeaman, Matsuda, Rossé, Camonis: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).

  • Target
    SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))
    Alternative Name
    SH3BP1 (SH3BP1 Products)
    Synonyms
    ARHGAP43 antibody, 3BP-1 antibody, SH3BP1 antibody, SH3 domain binding protein 1 antibody, SH3-domain binding protein 1 antibody, SH3 domain-binding protein 1 antibody, SH3BP1 antibody, Sh3bp1 antibody, LOC100398528 antibody
    Background
    SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
    Gene ID
    23616
    NCBI Accession
    NP_061830
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