Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (C-Term) antibody

Details for Product No. ABIN185194
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Antigen
Synonyms cpk, pi3-k-c2a, PIK3C2A, DKFZp469H1410, Cpk-m, PI3KC2, CPK, PI3-K-C2(ALPHA), PI3-K-C2A
Epitope
C-Term
(11), (7), (7), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(33), (2), (1)
Host
Goat
(22), (6), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(27), (23), (11), (7), (3), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
Options
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Catalog No. ABIN185194
374.27 $
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Immunogen Peptide with sequence C-TVKWYQLTAATYL, from the C Terminus of the protein sequence according to NP_002636.2.
Cross-Reactivity Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PIK3C2A
Background Synonyms: PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(ALPHA), phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase, DKFZp686L193, MGC142218
Gene ID 5286, 18704
NCBI Accession NP_002636
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1 µg/mL.
IHC: In paraffin embedded Human Heart shows strong staining of the capillary network and patterned staining of the myocardial fibres in transverse section. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (C-Term) antibody ABIN185194 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Molz, Chen, Hirano et al.: "Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13892-9, 1996 (PubMed).

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