Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (C-Term) antibody

Details for Product No. ABIN185194
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Antigen
Synonyms CPK, PI3-K-C2(ALPHA), PI3-K-C2A, Cpk-m, PI3KC2, cpk, pi3-k-c2a, PIK3C2A, DKFZp469H1410
Epitope
C-Term
(4), (2), (2), (1), (1), (1)
Reactivity
Human
(21), (4), (1)
Host
Goat
(11), (7), (5)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(15), (15), (12), (4), (3), (1), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN185194
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-TVKWYQLTAATYL, from the C Terminus of the protein sequence according to NP_002636.2.
Cross-Reactivity Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PIK3C2A
Background Synonyms: PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(ALPHA), phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase, DKFZp686L193, MGC142218
Gene ID 5286, 18704
NCBI Accession NP_002636.2
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1 µg/mL.
IHC: In paraffin embedded Human Heart shows strong staining of the capillary network and patterned staining of the myocardial fibres in transverse section. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (C-Term) antibody ABIN185194 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Molz, Chen, Hirano et al.: "Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13892-9, 1996 (PubMed).

Hosts (11), (7), (5)
Reactivities (21), (4), (1)
Applications (15), (15), (12), (4), (3), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1), (1)
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