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Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3) (C-Term) antibody

Details for Product No. ABIN185223
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Antigen
Synonyms DDBDRAFT_0184576, DDBDRAFT_0201632, DDB_0184576, DDB_0201632, arc21, p21-arc, 1110006A04Rik, 21kDa, AA408672, AI788639, p21-Ar, p21Arc, zgc:86828, ARC21, p21-Arc
Epitope
C-Term
(4), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(28), (7), (6), (2), (2), (1), (1), (1), (1), (1)
Host
Goat
(23), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(29), (16), (9), (4), (1)
Pubmed 2 references available
Quantity 100 µg
Options
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Catalog No. ABIN185223
374.27 $
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Immunogen Peptide with sequence C-RQFMNKSLSGPGQ, from the C Terminus of the protein sequence according to NP_005710.1.
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ARPC3
Background Synonyms: ARPC3, ARC21, p21-Arc, actin related protein 2/3 complex, subunit 3, 21kDa, ARP2/3 protein complex subunit p21, actin related protein 2/3 complex subunit 3
Gene ID 10094, 56378
NCBI Accession NP_005710.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Band observed just below 20 kDa marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20 kDa according to NP_005710). Recommended for use at 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3) (C-Term) antibody ABIN185223 staining (1µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product cited in: Fu, Brown, Yap et al.: "Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 709-21, 2013 (PubMed).

General Welch, DePace, Verma et al.: "The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly." in: The Journal of cell biology, Vol. 138, Issue 2, pp. 375-84, 1997 (PubMed).

Validation Images
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