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CD274 Molecule (CD274) (C-Term) antibody

Details for Product No. ABIN185339
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Antigen
Synonyms B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, B7h1, Pdl1, Pdcd1l1, Pdcd1lg1, A530045L16Rik, RGD1566211
Epitope
C-Term
(14), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(101), (97), (51), (20), (20)
Host
Goat
(79), (40), (36), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(18), (11), (11), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(89), (65), (45), (27), (24), (19), (15), (7), (5), (4), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 µg
Options
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Availability Available
Catalog No. ABIN185339
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CD274 / PD-L1
Background Synonyms: CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635, CD274, CD274 molecule, MGC142294, MGC142296
Gene ID 29126
NCBI Accession NP_054862.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35 kDa band observed in Human Heart lysates (calculated MW of 33.3 kDa according to NP_054862.1). Recommended concentration: 0.3-1.0 µg/mL.
IHC: In paraffin embedded Human Skel,etal Muscle shows staining of the striated muscular fibres. Recommended concentration: 4-6 µg/mL. Data obtained with a previous batch.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-CD274 Molecule (CD274) (C-Term) antibody ABIN185339 staining (0.3µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product cited in: Chen, Li, Meng et al.: "Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas." in: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 1, pp. 101-8, 2012 (PubMed).

General Thompson, Gillett, Cheville et al.: "Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 49, pp. 17174-9, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185339
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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