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PD-L1 antibody (CD274 Molecule) (C-Term)

Details for Product anti-CD274 Antibody No. ABIN185339, Supplier: Log in to see
Antigen
  • A530045L16Rik
  • B7h1
  • Pdcd1l1
  • Pdcd1lg1
  • Pdl1
  • B7-H
  • B7H1
  • PD-L1
  • PDCD1L1
  • PDCD1LG1
  • PDL1
  • RGD1566211
Epitope
C-Term
37
11
10
7
7
6
6
5
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
Reactivity
Human
192
93
21
1
1
Host
Goat
96
90
59
18
2
Clonality
Polyclonal
Conjugate
This PD-L1 antibody is un-conjugated
23
19
19
12
6
3
3
3
2
2
2
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
126
115
79
42
16
15
15
8
6
4
3
3
2
2
2
2
2
2
2
1
1
Supplier
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Immunogen Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1.
Blocking Peptide Blocking peptide for this product available: ABIN369296
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CD274 / PD-L1 (CD274 Antibody Abstract)
Background Synonyms: CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635, CD274, CD274 molecule, MGC142294, MGC142296
Gene ID 29126
NCBI Accession NP_054862
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35 kDa band observed in Human Heart lysates (calculated MW of 33.3 kDa according to NP_054862.1). Recommended concentration: 0.3-1.0 µg/mL.
IHC: In paraffin embedded Human Skel,etal Muscle shows staining of the striated muscular fibres. Recommended concentration: 4-6 µg/mL. Data obtained with a previous batch.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-PD-L1 antibody (CD274 Molecule) (C-Term) (ABIN185339) ABIN185339 staining (0.3µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein...
Product cited in: Chen, Li, Meng et al.: "Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas." in: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 1, pp. 101-8, 2012 (PubMed).

Background publications Thompson, Gillett, Cheville et al.: "Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 49, pp. 17174-9, 2004 (PubMed).