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Chemokine (C-C Motif) Ligand 3 (CCL3) (C-Term) antibody

Details for Product No. ABIN185352, Supplier: Login to see
Antigen
  • MIP-1alpha
  • G0S19-1
  • LD78ALPHA
  • MIP-1-alpha
  • MIP1A
  • SCYA3
  • MIP-1a
  • Scya3
  • AI323804
  • LD78alpha
  • MIP1-(a)
  • MIP1-alpha
  • Mip1a
  • CCL3L1
  • CCL3L3
Epitope
C-Term
21
20
5
5
5
4
3
3
2
2
2
2
2
2
1
1
1
Reactivity
Human
173
57
53
3
Host
Goat
148
84
32
7
4
Clonality
Polyclonal
Conjugate
Un-conjugated
35
10
9
7
7
7
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
201
191
30
23
21
18
15
12
7
7
6
5
5
5
2
2
1
1
Supplier
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Immunogen Peptide with sequence C-EWVQKYVSDLELSA, from the C Terminus of the protein sequence according to NP_002974.1, NP_066286.1.
Blocking Peptide Blocking peptide for this product available: ABIN369083
Specificity Please note this product is expected to recognize the products of 2 different genes which are almost identical.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CCL3 and CCL3L1 (CCL3 Antibody Abstract)
Background Synonyms: CCL3, chemokine (C-C motif) ligand 3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3, LD78 alpha beta, Small inducible cytokine A3, small inducible cytokine A3 (homologous to mouse Mip-1a), CCL3L1, LD78, 464.2, SCYA3L, G0S19-2, SCYA3L1, D17S1718, LD78BETA, MGC12815, chemokine (C-C motif) ligand 3-like 1, small inducible cytokine A3-like 1, MIP-1aP, MGC104178, MGC182017, MIP1AP
Gene ID 6348, 6349
NCBI Accession NP_066286, NP_002974
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 26+28 kDa (doublet) and 40 kDa in Human Bone Marrow and Spleen lysates after 0.1 µg/mL antibody staining.The 26+28 kDa doublet was also found in Human Lymph Node and in Tonsil lysates. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 10.2 kDa according to NP_066286.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain these bands).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Gonzalez, Kulkarni, Bolivar et al.: "The influence of CCL3L1 gene-containing segmental duplications on HIV-1/AIDS susceptibility." in: Science (New York, N.Y.), Vol. 307, Issue 5714, pp. 1434-40, 2005 (PubMed).