Apolipoprotein E (APOE) (C-Term) antibody

Details for Product No. ABIN185375
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Antigen
Synonyms AD2, LDLCQ5, LPG, AI255918, APOEA, Apo-E, ad2, apoprotein, im:7036787, wu:fb69a05, zgc:110064, apoe
Epitope
C-Term
(12), (5), (4), (3), (2), (1), (1)
Reactivity
Human
(170), (41), (31), (5), (4), (2), (2), (2), (1)
Host
Goat
(101), (64), (51), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (7), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(157), (130), (50), (41), (20), (18), (16), (12), (11), (9), (8), (7), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN185375
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-VGTSAAPVPSDNH, from the C Terminus of the protein sequence according to NP_000032.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name APOE
Background Synonyms: APOE, AD2, MGC1571, apoprotein, apolipoprotein E, apolipoprotein E3, Alzheimer disease 2 (APOE*E4-associated, late onset), LPG
Gene ID 348
NCBI Accession NP_000032.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 36 kDa band observed in Human Brain lysates (calculated MW of 36.2 kDa according to NP_000032.1). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Liver shows extra-cellular staining between hepatocytes. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Apolipoprotein E (APOE) (C-Term) antibody ABIN185375 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Apolipoprotein E (APOE) (C-Term) antibody (2) ABIN185375 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Dodart, Marr, Koistinaho et al.: "Gene delivery of human apolipoprotein E alters brain Abeta burden in a mouse model of Alzheimer's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 4, pp. 1211-6, 2005 (PubMed).

Levi, Jongen-Relo, Feldon et al.: "Brain area- and isoform-specific inhibition of synaptic plasticity by apoE4." in: Journal of the neurological sciences, Vol. 229-230, pp. 241-8, 2005 (PubMed).

Hosts (101), (64), (51), (1)
Reactivities (170), (41), (31), (5), (4), (2), (2), (2), (1)
Applications (157), (130), (50), (41), (20), (18), (16), (12), (11), (9), (8), (7), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Conjugates (13), (7), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (12), (5), (4), (3), (2), (1), (1)
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