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APOE antibody (Apolipoprotein E) (C-Term)

Details for Product anti-APOE Antibody No. ABIN185375, Supplier: Login to see
Antigen
  • ad2
  • apoprotein
  • im:7036787
  • wu:fb69a05
  • zgc:110064
  • apoe
  • AI255918
  • AD2
  • LDLCQ5
  • LPG
  • APOEA
  • Apo-E
Epitope
C-Term
33
13
6
6
5
5
4
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
276
64
34
8
5
2
2
1
Host
Goat
144
110
77
Clonality
Polyclonal
Conjugate
This APOE antibody is un-conjugated
24
19
12
5
5
5
3
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
230
191
71
57
19
16
15
14
11
8
7
6
5
4
3
2
2
2
2
2
2
2
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-VGTSAAPVPSDNH, from the C Terminus of the protein sequence according to NP_000032.1.
Blocking Peptide Blocking peptide for this product available: ABIN370017
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name APOE (APOE Antibody Abstract)
Background Synonyms: APOE, AD2, MGC1571, apoprotein, apolipoprotein E, apolipoprotein E3, Alzheimer disease 2 (APOE*E4-associated, late onset), LPG
Gene ID 348
NCBI Accession NP_000032
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 36 kDa band observed in Human Brain lysates (calculated MW of 36.2 kDa according to NP_000032.1). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Liver shows extra-cellular staining between hepatocytes. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-APOE antibody (Apolipoprotein E) (C-Term) (ABIN185375) ABIN185375 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). P...
Immunohistochemistry (IHC) image for anti-APOE antibody (Apolipoprotein E) (C-Term) (ABIN185375) ABIN185375 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retr...
Background publications Levi, Jongen-Relo, Feldon et al.: "Brain area- and isoform-specific inhibition of synaptic plasticity by apoE4." in: Journal of the neurological sciences, Vol. 229-230, pp. 241-8, 2005 (PubMed).

Dodart, Marr, Koistinaho et al.: "Gene delivery of human apolipoprotein E alters brain Abeta burden in a mouse model of Alzheimer's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 4, pp. 1211-6, 2005 (PubMed).