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Details for Product No. ABIN185377

Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (C-Term) antibody

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Synonyms CD32, FCG2, CD32B, FCGR2, IGFR2, FCGR2B
»Alternatives C-Term
»Alternatives Human
Clonality Polyclonal
»Alternatives Un-conjugated
Methode Type Peptide ELISA
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN185377
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus of the protein sequence according to NP_003992.3, NP_001002273.1, NP_001002274.1, NP_001002275.1, NP_001177757.1.
Specificity This antibody is expected to recognise all reported isoforms (NP_001002273.1, NP_001002274.1, NP_001002275.1, NP_003992.3, NP_001177757.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CD32 / FCGR2B
Background Synonyms: FCGR2B, CD32, FCG2, FCGR2, IGFR2, Fc fragment of IgG, low affinity IIb, receptor for (CD32), Fc gamma RIIb, Fc fragment of IgG, low affinity II, receptor for (CD32), Fc fragment of IgG, low affinity IIb, receptor (CD32), CD32B, Fc fragment of IgG, low affinity IIb, receptor
Gene ID 2213
NCBI Accession NP_003992.3, NP_001002273.1, NP_001002274.1, NP_001002275.1, NP_001177757.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 34 kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34.0 kDa according to NP_003992.3,). In transfected HEK293 transiently expressing CD32 a band of approx. 40 kDa (glycoprotein) is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Liver shows membranous staining in endothelial cells between the hepatocytes. Similar results were obtained in Human Placenta. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Hitomi, Tsuchiya, Kawasaki et al.: "CD72 polymorphisms associated with alternative splicing modify susceptibility to human systemic lupus erythematosus through epistatic interaction with FCGR2B." in: Human molecular genetics, Vol. 13, Issue 23, pp. 2907-17, 2004 (PubMed).

McGaha, Sorrentino, Ravetch: "Restoration of tolerance in lupus by targeted inhibitory receptor expression." in: Science (New York, N.Y.), Vol. 307, Issue 5709, pp. 590-3, 2005 (PubMed).

Alternatives for antigen "Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B)", type "Antibodies"
Hosts (31), (9), (4), (2)
Reactivities (41), (18), (14), (13), (13), (12), (1)
Applications (30), (17), (10), (9), (4), (4), (3), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (12), (10), (4), (2), (2), (2), (1), (1), (1)