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Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (C-Term) antibody

Details for Product No. ABIN185377, Supplier: Log in to see
Antigen
  • CD32
  • CD32B
  • FCG2
  • FCGR2
  • IGFR2
  • FCGR2B
  • AI528646
  • F630109E10Rik
  • Fc[g]RII
  • FcgRII
  • Fcgr2
  • Fcgr2a
  • Fcr-2
  • Fcr-3
  • Ly-17
  • Ly-m20
  • LyM-1
  • Lym-1
Epitope
C-Term
27
18
15
13
5
3
3
3
3
3
3
2
2
2
Reactivity
Human
68
27
22
4
4
1
1
1
Host
Goat
60
14
5
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
50
38
12
11
9
5
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus of the protein sequence according to NP_003992.3, NP_001002273.1, NP_001002274.1, NP_001002275.1, NP_001177757.1.
Blocking Peptide Blocking peptide for this product available: ABIN369531
Specificity This antibody is expected to recognise all reported isoforms (NP_001002273.1, NP_001002274.1, NP_001002275.1, NP_003992.3, NP_001177757.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CD32 / FCGR2B (FCGR2B Antibody Abstract)
Background Synonyms: FCGR2B, CD32, FCG2, FCGR2, IGFR2, Fc fragment of IgG, low affinity IIb, receptor for (CD32), Fc gamma RIIb, Fc fragment of IgG, low affinity II, receptor for (CD32), Fc fragment of IgG, low affinity IIb, receptor (CD32), CD32B, Fc fragment of IgG, low affinity IIb, receptor
Gene ID 2213
NCBI Accession NP_001002274, NP_001002273, NP_001177757, NP_001002275, NP_003992
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 34 kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34.0 kDa according to NP_003992.3,). In transfected HEK293 transiently expressing CD32 a band of approx. 40 kDa (glycoprotein) is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Liver shows membranous staining in endothelial cells between the hepatocytes. Similar results were obtained in Human Placenta. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (C-Term) antibody (ABIN185377) ABIN185377 (0.3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary ...
Background publications McGaha, Sorrentino, Ravetch: "Restoration of tolerance in lupus by targeted inhibitory receptor expression." in: Science (New York, N.Y.), Vol. 307, Issue 5709, pp. 590-3, 2005 (PubMed).

Hitomi, Tsuchiya, Kawasaki et al.: "CD72 polymorphisms associated with alternative splicing modify susceptibility to human systemic lupus erythematosus through epistatic interaction with FCGR2B." in: Human molecular genetics, Vol. 13, Issue 23, pp. 2907-17, 2004 (PubMed).