Cholecystokinin B Receptor (CCKBR) (Internal Region) antibody

Details for Product No. ABIN185407
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Antigen
Synonyms CCKBR, LOC100232835, CCK-B, CCK2R, GASR, Cck2r, Cholrec, CCKR-2, CCK-BR, CCK2-R, CCK-CHR
Epitope
Internal Region
(6), (5), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(63), (30), (29), (16), (1), (1)
Host
Goat
(58), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(45), (24), (21), (11), (10), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 100 μg
Options
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Catalog No. ABIN185407
374.27 $
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Immunogen Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1.
Cross-Reactivity Mouse (Murine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CCKBR
Background Synonyms: CCKBR, cholecystokinin B receptor, HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrincholecystokinin brain receptor, CCK2R
Gene ID 887, 12426, 25706
NCBI Accession NP_795344
Research Area Neurology, Endocrine system
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 75 kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4 kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J, Int J Endocrinol. 2009,2009:875167, PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov,51(11):1501-13, PMID: 14566022). Data obtained from antibodies from a previous goat. Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Pancreas shows staining of the islets of Langerhans. Recommended concentration: 4-6 µg/mL. Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for RIN-14B cells as described in the following paper: El-Kouhen et al, Pancreas. 2010 Mar,39(2):127-34, PMID: 19959964 and for rat pancreatic stellate cells as described in: Berna et al, J Biol Chem. 2010 Dec 10,285(50):38905-14, PMID: 20843811. Data obtained from antibodies from a previous goat.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Cholecystokinin B Receptor (CCKBR) (Internal Region) antibody ABIN185407 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Product cited in: El-Kouhen, Morisset: "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line." in: Pancreas, Vol. 39, Issue 2, pp. 127-34, 2010 (PubMed).

El-Kouhen, Morisset: "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells." in: International journal of endocrinology, Vol. 2009, pp. 875167, 2009 (PubMed).

Berna, Seiz, Nast et al.: "CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 38905-14, 2010 (PubMed).

Quattrone, Dewaele, Wozniak et al.: "Promoting role of cholecystokinin 2 receptor (CCK2R) in gastrointestinal stromal tumour pathogenesis." in: The Journal of pathology, 2012 (PubMed).

General Olszewska-Pazdrak, Townsend, Hellmich: "Agonist-independent activation of Src tyrosine kinase by a cholecystokinin-2 (CCK2) receptor splice variant." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40400-4, 2004 (PubMed).

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