Pancreatic Polypeptide (PPY) (Internal Region) antibody

Details for Product No. ABIN185420
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Antigen
Synonyms PPY, PNP, PP, AV061413, IGSPP
Epitope
Internal Region
(4), (4), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(43), (19), (18), (17), (17), (13), (2), (1)
Host
Goat
(40), (8), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(25), (22), (16), (13), (10), (6), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185420
374.27 $
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  • +1 404 474 4654
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Immunogen Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Pancreatic Polypeptide / PPY
Background Synonyms: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide Y
Gene ID 5539
NCBI Accession NP_002713
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa according to NP_002713.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3 µg/mL. An anonymous customer found positive results in IHC on Mouse pancreas.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Pancreatic Polypeptide (PPY) (Internal Region) antibody ABIN185420 (3µg/ml) staining of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
General Badman, Flier: "The gut and energy balance: visceral allies in the obesity wars." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1909-14, 2005 (PubMed).

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