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Details for Product No. ABIN185420

Pancreatic Polypeptide (PPY) (Internal Region) antibody

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Antigen
Synonyms PNP, AV061413, IGSPP, PP, PPY
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human, Mouse (Murine)
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN185420
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Pancreatic Polypeptide / PPY
Background Synonyms: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide Y
Gene ID 5539
NCBI Accession NP_002713.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa according to NP_002713.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3 µg/mL. An anonymous customer found positive results in IHC on Mouse pancreas.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Badman, Flier: "The gut and energy balance: visceral allies in the obesity wars." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1909-14, 2005 (PubMed).

Alternatives for antigen "Pancreatic Polypeptide (PPY)", type "Antibodies"
Hosts (28), (8), (6)
Reactivities (31), (6), (6), (5), (5), (2), (1), (1)
Applications (22), (18), (8), (7), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Epitopes (4), (2), (1)
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