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BR serine/threonine Kinase 2 (BRSK2) (Internal Region) antibody

Details for Product No. ABIN185503
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Antigen
Synonyms BRSK2, C11orf7, PEN11B, SAD1, SADA, STK29, 4833424K13Rik, SAD-A
Epitope
Internal Region
(15), (7), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
(39), (31), (16), (13), (12), (12), (12)
Host
Goat
(43), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(35), (25), (17), (10), (7), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN185503
261.61 $
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Immunogen Peptide with sequence CPEVIRGEKYDGRKAD, from the internal region of the protein sequence according to NP_003948.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BRSK2 / STK29
Background Synonyms: BRSK2, SAD1, STK29, PEN11B, C11orf7, BR serine/threonine kinase 2, serine/threonine kinase 29, homolog of SAD-1 (C. elegans), chromosome 11 open reading frame 7, chromsosome 11 open reading frame 7, FLJ41362, protein kinase SAD1B, serine/threonine kinase SAD-A
Gene ID 9024, 75770, 293631
NCBI Accession NP_003948.2
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave bands at approx 50-55 kDa and 15 kDa in human brain, mouse brain and rat brain lysates after 0.5 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.7 kDa according to NP_003948.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Kishi, Pan, Crump et al.: "Mammalian SAD kinases are required for neuronal polarization." in: Science (New York, N.Y.), Vol. 307, Issue 5711, pp. 929-32, 2005 (PubMed).

Validation Images
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