BRSK2 antibody (C-Term)

Catalog No. ABIN391285

This Rabbit Polyclonal antibody specifically detects BRSK2 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for BRSK2 antibody (C-Term) (ABIN391285)

Target: BRSK2 (BR serine/threonine Kinase 2 (BRSK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRSK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB5548

Product Details anti-BRSK2 Antibody

Binding Specificity: AA 706-736, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This STK29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-736 amino acids from the C-terminal region of human STK29.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for BRSK2

Target: BRSK2 (BR serine/threonine Kinase 2 (BRSK2))

Alternative Name: STK29

Background: BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C, and CDC25B in an in vitro assay, but a kinase-dead mutant does not. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.

Molecular Weight: 81633

Gene ID: 9024

NCBI Accession: NP_001243556, NP_001243558, NP_001243559, NP_003948

UniProt: Q8IWQ3