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Tripartite Motif Containing 5 (TRIM5) (Internal Region) antibody

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Synonyms TRIM5, RNF88, TRIM5alpha, EG667823, Gm8833, RGD1304579
Internal Region
(41), (21), (15), (13), (6), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(131), (6), (2), (1), (1), (1)
(85), (37), (14)
(4), (4), (4), (4), (4), (4), (1)
Methode Type Peptide ELISA
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(114), (88), (55), (26), (7), (6), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence CNIEKNENYQPK, from the internal region of the protein sequence according to NP_149023.1.
Blocking Peptide Blocking peptide for this product available: ABIN369467
Specificity This antibody is expected to recognise isoform alpha (NP_149023.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TRIM5 / RNF88 (alpha) (TRIM5 Antibody Abstract)
Background Synonyms: TRIM5, tripartite motif-containing 5, HGNC:16276, RNF88, tripartite motif protein 5, tripartite motif protein TRIM5, TRIM5alpha, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Gene ID 85363
NCBI Accession NP_149023
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 37 kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3 kDa according to NP_149023.1. The 37 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Berthoux, Sebastian, Sokolskaja et al.: "Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 41, pp. 14849-53, 2005 (PubMed).

Catalog No. ABIN185553
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