Tripartite Motif Containing 5 (TRIM5) (Internal Region) antibody

Details for Product No. ABIN185553
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Antigen
Synonyms TRIM5, RNF88, TRIM5alpha, EG667823, Gm8833, RGD1304579
Epitope
Internal Region
(37), (8), (7), (7), (4), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Reactivity
Human
(119), (6), (2)
Host
Goat
(91), (20), (13)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(108), (78), (42), (31), (10), (10), (3), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185553
374.27 $
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Immunogen Peptide with sequence CNIEKNENYQPK, from the internal region of the protein sequence according to NP_149023.1.
Specificity This antibody is expected to recognise isoform alpha (NP_149023.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TRIM5 / RNF88 (alpha)
Background Synonyms: TRIM5, tripartite motif-containing 5, HGNC:16276, RNF88, tripartite motif protein 5, tripartite motif protein TRIM5, TRIM5alpha, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Gene ID 85363
NCBI Accession NP_149023
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 37 kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3 kDa according to NP_149023.1. The 37 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Berthoux, Sebastian, Sokolskaja et al.: "Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 41, pp. 14849-53, 2005 (PubMed).

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