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Tripartite Motif Containing 5 (TRIM5) (Internal Region) antibody

Details for Product No. ABIN185553, Supplier: Login to see
Antigen
  • TRIM5
  • RNF88
  • TRIM5alpha
  • EG667823
  • Gm8833
  • RGD1304579
Epitope
Internal Region
41
21
15
13
6
4
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
131
6
2
1
1
1
Host
Goat
85
37
14
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
4
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
114
88
55
26
7
6
1
1
1
1
1
Supplier
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Immunogen Peptide with sequence CNIEKNENYQPK, from the internal region of the protein sequence according to NP_149023.1.
Blocking Peptide Blocking peptide for this product available: ABIN369467
Specificity This antibody is expected to recognise isoform alpha (NP_149023.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TRIM5 / RNF88 (alpha) (TRIM5 Antibody Abstract)
Background Synonyms: TRIM5, tripartite motif-containing 5, HGNC:16276, RNF88, tripartite motif protein 5, tripartite motif protein TRIM5, TRIM5alpha, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Gene ID 85363
NCBI Accession NP_149023
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 37 kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3 kDa according to NP_149023.1. The 37 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Berthoux, Sebastian, Sokolskaja et al.: "Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 41, pp. 14849-53, 2005 (PubMed).