Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibody

Details for Product No. ABIN185719
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Antigen
Synonyms XLF, 1700029B21Rik, cernunnos, NHEJ1, zgc:85657
Epitope
C-Term
(8), (2), (1)
Reactivity
Human
(33), (4), (4), (3), (2)
Host
Goat
(16), (14), (5)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(34), (14), (12), (7), (7), (4), (2)
Pubmed 1 reference available
Catalog no. ABIN185719
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Alternative Name XRCC4-like factor / NHEJ1
Background Synonyms: NHEJ1, XRCC4-like factor, nonhomologous end-joining factor 1, FLJ12610, XLF, Cernunnos
Gene ID 79840
NCBI Accession NP_079058.1
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38 kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3 kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration: 2.5-3.8 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibody ABIN185719 (0.1 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Ahnesorg, Smith, Jackson: "XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining." in: Cell, Vol. 124, Issue 2, pp. 301-13, 2006 (PubMed).

Hosts (16), (14), (5)
Reactivities (33), (4), (4), (3), (2)
Applications (34), (14), (12), (7), (7), (4), (2)
Epitopes (8), (2), (1)
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