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Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibody

Details for Product No. ABIN185719, Supplier: Login to see
Antigen
  • NHEJ1
  • zgc:85657
  • XLF
  • 1700029B21Rik
  • cernunnos
Alternatives
anti-Human Nonhomologous End-Joining Factor 1 antibody for ELISA
Epitope
C-Term
37
15
3
2
2
2
1
Reactivity
Human
87
6
6
5
3
Host
Goat
39
32
17
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
79
31
27
19
13
12
10
2
1
Supplier
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Immunogen Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.
Blocking Peptide Blocking peptide for this product available: ABIN369699
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Alternative Name XRCC4-like factor / NHEJ1 (NHEJ1 Antibody Abstract)
Background Synonyms: NHEJ1, XRCC4-like factor, nonhomologous end-joining factor 1, FLJ12610, XLF, Cernunnos
Gene ID 79840
NCBI Accession NP_079058
Pathways DNA Damage Repair
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38 kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3 kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration: 2.5-3.8 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibody (ABIN185719) ABIN185719 (0.1 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer...
Background publications Ahnesorg, Smith, Jackson: "XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining." in: Cell, Vol. 124, Issue 2, pp. 301-13, 2006 (PubMed).