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Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibody

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Synonyms NHEJ1, zgc:85657, XLF, 1700029B21Rik, cernunnos
(37), (15), (3), (2), (2), (2), (1)
(87), (6), (6), (5), (3)
(39), (32), (17)
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(79), (31), (27), (19), (13), (12), (10), (2), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.
Blocking Peptide Blocking peptide for this product available: ABIN369699
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Alternative Name XRCC4-like factor / NHEJ1 (NHEJ1 Antibody Abstract)
Background Synonyms: NHEJ1, XRCC4-like factor, nonhomologous end-joining factor 1, FLJ12610, XLF, Cernunnos
Gene ID 79840
NCBI Accession NP_079058
Pathways DNA Damage Repair
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38 kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3 kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration: 2.5-3.8 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibody (ABIN185719) ABIN185719 (0.1 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer...
Background publications Ahnesorg, Smith, Jackson: "XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining." in: Cell, Vol. 124, Issue 2, pp. 301-13, 2006 (PubMed).

Catalog No. ABIN185719
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