Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1) (Internal Region) antibody

Details for Product No. ABIN190770
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Antigen
Synonyms CACNA2D1, DKFZp469K2211, CACNA2, CACNL2A, CCHL2A, Ca(v)alpha2delta1, Cacna2, Cchl2a, CCHLA2, DHSCCA
Epitope
Internal Region
(14), (3), (2), (2), (1), (1), (1)
Reactivity
Human
(52), (33), (31), (20), (19), (18), (3), (1), (1)
Host
Goat
(49), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(40), (21), (18), (14), (8), (8), (2), (1), (1)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN190770
374.27 $
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Immunogen Peptide with sequence C-QEIFNKYNKDKKVR, from the internal region of the protein sequence according to NP_000713.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name CACNA2D1
Background Synonyms: CACNA2D1, calcium channel, voltage-dependent, alpha 2/delta subunit 1, CACNA2, CACNL2A, CCHL2A, MHS3, H_DJ0560O14.1, WUGSC:H_DJ0560O14.1, calcium channel, L type, alpha 2 polypeptide, dihydropyridine-sensitive L-type, calcium channel alpha-2/delta subunit
Gene ID 781, 12293, 25399
NCBI Accession NP_000713
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 140 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 123 kDa according to NP_000713.2). Recommended concentration: 0.03-0.1 µg/mL. Customer found it not suitable on Mouse and Rat protein.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1) (Internal Region) antibody ABIN190770 (0.03µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product cited in: Neely, Hess, Costigan et al.: "A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene." in: Cell, Vol. 143, Issue 4, pp. 628-38, 2010 (PubMed).

General Hullin, Matthes, von Vietinghoff et al.: "Increased expression of the auxiliary beta(2)-subunit of ventricular L-type Ca(2)+ channels leads to single-channel activity characteristic of heart failure." in: PLoS ONE, Vol. 2, Issue 3, pp. e292, 2007 (PubMed).

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