Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Internal Region) antibody

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Synonyms APPL, DIP13alpha, 2900057D21Rik, 7330406P05Rik, AI585782, AW209077, BB022931, C88264, DIP13, RGD1309388
Internal Region
(14), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
(57), (25), (14), (3), (3), (1), (1), (1), (1), (1)
(43), (15), (2)
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(58), (35), (14), (7), (4), (3), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Availability Available
Immunogen Peptide with sequence C-NRYSRLSKKRENDK, from the internal region of the protein sequence according to NP_036228.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name APPL1 (APPL1 Antibody Abstract)
Background Synonyms: APPL1, DIP13alpha, adaptor protein containing pH domain, PTB domain and leucine zipper motif, signaling adaptor protein DIP13alpha
Gene ID 26060, 72993
NCBI Accession NP_036228
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 80 kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7 kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Internal Region) antibody (ABIN190879) ABIN190879 (0.5µg/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after in...
Background publications Mao, Kikani, Riojas et al.: "APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function." in: Nature cell biology, Vol. 8, Issue 5, pp. 516-23, 2006 (PubMed).

Catalog No. ABIN190879
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