APPL1 antibody (AA 467-516)

Catalog No. ABIN6747166

This Rabbit Polyclonal antibody specifically detects APPL1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Monkey and Pig.

Quick Overview for APPL1 antibody (AA 467-516) (ABIN6747166)

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APPL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APPL1 Antibody

Binding Specificity: AA 467-516

Specificity: Human APPL1

Predicted Reactivity: Percent identity by BLAST analysis: Rat, Guinea pig (100%) Dog, Horse (92%) Bovine, Pig, Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa467-516 of human APPL1 (Q9UKG1, NP_036228). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Guinea pig (100%), Elephant, Dog, Bat, Horse, Lizard (92%), Bovine, Pig, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for APPL1

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Alternative Name: APPL1 / APPL

Background: Name/Gene ID: APPL1

Synonyms: APPL1, Dip13-alpha, DIP13A, DIP13alpha, AKT2 interactor, KIAA1428, APPL

Gene ID: 26060

NCBI Accession: NP_036228

UniProt: Q9UKG1