CD2 antibody (FITC)

Catalog No. ABIN2144452

Product Details anti-CD2 Antibody (FITC)

Target: CD2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Characteristics: This antibody recognizes a 50KDa lymphocyte surface antigen which is expressed on all peripheral blood T lymphocytes,the majority of lymphocytes and malignant cells of T cell origin, including T ALL cells. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. CD2 antigen has been characterised as the receptor for sheep erythrocytes. This CD2 monoclonal antibody inhibits E rosette formation. CD2 antigen also functions as the receptor for the CD58 antigen(LFA-3).

Clone: 4ARPA-2-10

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD2

Target: CD2

Alternative Name: CD2 (CD2 Products)