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- Target See all CD33 Antibodies
- CD33
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD33 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Characteristics
- 4A6C5 reacts with CD33 andtigen, a 67 kDa type I transmembrane glycoprotein present on myeloid progenitors, monocytes andgranulocytes. CD33 is absent on lymphocytes, platelets, erythrocytes , hematopoietic stem cells and non-hematopoietic cystem.. CD33 antigen can function as a sialic acid-dependent cell adhesion molecule and involved in negative selection of human self-regenerating hemetopoietic stem cells. This clone is cross reactive with non-human primate * Diagnosis of acute myelogenousnleukemia. Negative selection for human self-regenerating hematopoietic stem cells.
- Clone
- 4A6C5
- Isotype
- IgG1
- Top Product
- Discover our top product CD33 Primary Antibody
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anti-CD33 (CD33) (AA 101-200) antibody
CD33 Reactivity: Human, Mouse FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 15-237) antibodyCD33 Reactivity: Human FACS, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated
anti-CD33 (CD33) (AA 15-237) antibodyCD33 Reactivity: Human FACS, WB, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated
anti-CD33 (CD33) (AA 18-240) antibodyCD33 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 49-259) antibodyCD33 Reactivity: Human WB, IF, IHC Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 261-364) antibodyCD33 Reactivity: Human FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 1-364) antibodyCD33 Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 2D12-G4 unconjugated
anti-CD33 (CD33) antibodyCD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, CyTOF Host: Mouse Monoclonal WM53 unconjugated
anti-CD33 (CD33) antibodyCD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, CyTOF Host: Mouse Monoclonal WM53 unconjugated
anti-CD33 (CD33) antibody (Biotin)CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal HIM3-4 Biotin
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Procedure
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- CD33
- Alternative Name
- CD33 (CD33 Products)
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