CD147 antibody (PE)
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- Target See all CD147 (BSG) Antibodies
- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD147 antibody is conjugated to PE
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Characteristics
- 4AHIM6 monoclonal antibody reacts with basigin or neurothelin, a 50-60 kDa transmembrane glycoprotein, broadly expressed on cells of hematopoietic and non-hematopoietic origin. Neutrothelin is a blood-brain barrier-specific molecule. CD147 play a role in embryonal blood barrier development and a role in integrin-mediated adhesion in brain endothelia.
- Clone
- 4AHIM6
- Isotype
- IgG1
- Top Product
- Discover our top product BSG Primary Antibody
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anti-Basigin (Ok Blood Group) (BSG) (AA 79-280) antibody
BSG Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 87-323) antibodyBSG Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 59-321) antibodyBSG Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG Reactivity: Human FACS, IHC, ELISA Host: Mouse Monoclonal 2D8C11 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG Reactivity: Human FACS, IHC, ELISA Host: Mouse Monoclonal 7H4D4 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 30-200) antibodyBSG Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG Reactivity: Human, Mouse, Rat WB, FACS, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 3E10C1 unconjugated
anti-Basigin (Ok Blood Group) (BSG) antibodyBSG Reactivity: Human IHC, StM Host: Mouse Monoclonal BSG-963 unconjugated
anti-Basigin (Ok Blood Group) (BSG) antibodyBSG Reactivity: Human, Pig FACS, IHC (p) Host: Mouse Monoclonal MEM-M6-2 unconjugated
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Procedure
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
- Alternative Name
- CD147 (BSG Products)
- Pathways
- S100 Proteins
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