Mesothelin (MSLN) (Extracellular Domain) antibody

Antigen: Mesothelin (MSLN)

Synonyms: MPF, SMR, CAK1, MSLN

Binding Site: Extracellular Domain

Protein Type: Recombinant

Clonality: Monoclonal (MN-1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN233753

Additional Information

Alternative name: Mesothelin

Gene ID: 10232

UniProt: Q13421

Immunogen: This antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the of human mesothelin.

Format: Liquid

Isotype: IgG

Clone: MN-1

Description: Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Application Details

Application Notes: This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
ELISA Dilution: 1:10,000 - 1:50,000
Immunohistochemistry Dilution: 1:100
Western Blot Dilution: 1:1,000

Concentration: 1.0 mg/mL

Purification: Sterile filtered

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Research Area: Cancer

Restrictions: For Research Use only

Images

Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB, and D, MAb MN. Reprinted with permission fromClin.Cancer Res. 11(16):5840-6.

Immunohistochemistry using antibodies-online Immunochemicals anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB

Publications

Xiang, Phung, Feng et al.: "The Development and Characterization of a Human Mesothelioma In Vitro 3D Model to Investigate Immunotoxin Therapy." in: PLoS ONE, Vol. 6, Issue 1, pp. e14640, 2011 (PubMed).