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FAS antibody (Fas (TNF Receptor Superfamily, Member 6)) (PE)

Details for Product anti-FAS Antibody No. ABIN238439, Supplier: Login to see
Antigen
  • AI196731
  • APO1
  • APT1
  • CD95
  • TNFR6
  • Tnfrsf6
  • lpr
  • TNFRSF6
  • ALPS1A
  • APO-1
  • FAS1
  • FASTM
  • A630082H08Rik
  • FAS
Alternatives
anti-Human FAS antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human
657
106
84
11
7
6
4
3
3
3
2
2
1
1
Host
Mouse
434
297
12
7
6
5
3
Clonality (Clone)
Monoclonal ()
Conjugate
This FAS antibody is conjugated to PE
75
63
48
31
14
7
6
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
355
300
201
137
111
82
65
62
47
44
30
13
12
8
7
5
4
4
3
3
3
2
2
2
2
1
1
1
Supplier
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Immunogen HUT-78 human T cell lymphoma cell line.
Clone LT95
Isotype IgG1
Specificity The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Characteristics Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6
Purification Size-exclusion chromatography.
Alternative Name CD95 / FAS (FAS Antibody Abstract)
Background CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6
Gene ID 355
UniProt P25445
Pathways p53 Signaling, Apoptosis, TCR Signaling
Application Notes Suitable for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL ofwhole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline (PBS), 15 mM sodium azide, 0.2 % (w/v) high-grade BSA (Protease free)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Brumatti, Yon, Castro et al.: "Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide." in: Experimental cell research, Vol. 314, Issue 3, pp. 554-63, 2008 (PubMed).

Guo, Zhang, Tang et al.: "Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines." in: Blood, Vol. 106, Issue 6, pp. 2033-41, 2005 (PubMed).

Drosopoulos, Roberts, Cermak et al.: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, 2005 (PubMed).

Nakamura, Kakinuma, Amada et al.: "Pyrazole derivatives as new potent and selective 20-hydroxy-5,8,11,14-eicosatetraenoic acid synthase inhibitors." in: Bioorganic & medicinal chemistry, Vol. 12, Issue 23, pp. 6209-19, 2004 (PubMed).

Park, Thomsen, Frevert et al.: "Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6209-16, 2003 (PubMed).

Scaffidi, Fulda, Srinivasan et al.: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, 1998 (PubMed).