ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (Internal Region) antibody

Details for Product No. ABIN238543
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Antigen
Synonyms ASF1, ASF1A, CGI-98, CIA, HSPC146, 2310079C17Rik, C79052
Epitope
Internal Region
(10), (7), (6), (3), (1), (1), (1)
Reactivity
Human
(47), (27), (16), (12), (12), (4)
Host
Goat
(37), (11), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(38), (24), (10), (3), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
Options
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Catalog No. ABIN238543
374.27 $
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Immunogen Peptide with sequence C-EDNTEKLEDAE, from the internal region of the protein sequence according to NP_054753.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ASF1A / HSPC146
Background Synonyms: ASF1A, HSPC146, ASF1 anti-silencing function 1 homolog A (S. cerevisiae), RP3-329L24.1, CGI-98, CIA, DKFZP547E2110, ASF1 anti-silencing function 1 homolog A, CCG1-interacting factor A, anti-silencing function 1A
Gene ID 25842, 66403, 294408
NCBI Accession NP_054753
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx. 23 kDa band observed in lysates of cell line Jurkat (calculated MW of 23.0 kDa according to NP_054753.1). Recommended concentration: 0.01-0.03 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (Internal Region) antibody ABIN238543 (0.01µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Sanematsu, Takami, Barman et al.: "Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13817-27, 2006 (PubMed).

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