Corticotropin Releasing Hormone Receptor 1 (CRHR1) (Internal Region) antibody

Details for Product No. ABIN238557
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Antigen
Synonyms CRHR1, CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R-1, CRH-R1, CRH-R1h, CRHR, CRHR1L, CRHR1f, Crhr, crhr1, crhr1-A
Epitope
Internal Region
(13), (11), (8), (6), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(70), (23), (20), (4), (3), (2), (2), (2), (2), (2), (1)
Host
Goat
(59), (12), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(46), (36), (21), (18), (13), (5), (5), (4), (3), (2)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN238557
374.27 $
Plus shipping costs $45.00

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Immunogen Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.
Cross-Reactivity Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CRHR1 / CRF-R
Background Synonyms: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor
Gene ID 1394, 12921, 58959
NCBI Accession NP_004373
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60-70 kDa band observed in human brain (cerebellum) and human colon lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar,59(3):485-92, PMID: 11179443) . Recommended concentration: 0.1-0.3 µg/mL.
IHC: This product was successfully used on cryosection of mouse brain (PMID: 22570383) Immunofluorescence: This product was successfully used in immunofluorescence on mouse brain at 2 µg/mL when incubated at 4C for 40h.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (Internal Region) antibody ABIN238557 (0.1 µg/mL) staining of human colon lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (Internal Region) antibody (2) Confocal image (3 µm) of ABIN238557 (0.3 µg/mL) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.
Product cited in: Jüngling, Liu, Lesting et al.: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." in: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, 2012 (PubMed).

General Rissman, Lee, Vale et al.: "Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 24, pp. 6552-62, 2007 (PubMed).

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