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Corticotropin Releasing Hormone Receptor 1 (CRHR1) (Internal Region) antibody

Details for Product No. ABIN238557, Supplier: Log in to see
Antigen
  • CRHR1
  • CRF-R
  • CRF-R-1
  • CRF-R1
  • CRF1
  • CRFR-1
  • CRFR1
  • CRH-R-1
  • CRH-R1
  • CRH-R1h
  • CRHR
  • CRHR1L
  • CRHR1f
  • Crhr
  • crhr1
  • crhr1-A
Epitope
Internal Region
23
15
12
11
11
10
9
9
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
137
32
29
5
4
3
2
2
2
2
2
1
1
1
1
Host
Goat
103
32
6
Clonality
Polyclonal
Conjugate
Un-conjugated
13
13
13
6
6
4
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
84
69
32
23
22
21
3
2
1
1
Supplier
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Immunogen Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.
Blocking Peptide Blocking peptide for this product available: ABIN369451
Cross-Reactivity Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CRHR1 / CRF-R (CRHR1 Antibody Abstract)
Background Synonyms: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor
Gene ID 1394, 12921, 58959
NCBI Accession NP_004373
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60-70 kDa band observed in human brain (cerebellum) and human colon lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar,59(3):485-92, PMID: 11179443) . Recommended concentration: 0.1-0.3 µg/mL.
IHC: This product was successfully used on cryosection of mouse brain (PMID: 22570383) Immunofluorescence: This product was successfully used in immunofluorescence on mouse brain at 2 µg/mL when incubated at 4C for 40h.
Restrictions For Research Use only
Format Liquid
Concentration 0.5mg/ml
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (Internal Region) antibody (ABIN238557) ABIN238557 (0.1 µg/mL) staining of human colon lysate (35 µg protein in RIPA buffer)....
 image for anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (Internal Region) antibody (ABIN238557) Confocal image (3 µm) of ABIN238557 (0.3 µg/mL) staining in green a dendrite of a neu...
Product cited in: Jüngling, Liu, Lesting et al.: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." in: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, 2012 (PubMed).

Weerachayaphorn, Mennone, Soroka et al.: "Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 9, pp. G925-36, 2012 (PubMed).

Background publications Rissman, Lee, Vale et al.: "Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 24, pp. 6552-62, 2007 (PubMed).