CRHR1 antibody (Extracellular Domain)

Catalog No. ABIN1048462

Product Details anti-CRHR1 Antibody

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Binding Specificity: Extracellular Domain

Reactivity: Human, Dog, Monkey, Horse, Cow, Hamster, Sheep, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRHR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Sheep, Hamster, Dog, Bovine, Horse, Pig, Opossum (100%) Elephant, Panda, Bat (94%) Marmoset, Mouse, Rat, Seabass, Stickleback, Pufferfish (89%) Turkey, Chicken, Lizard, Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from 1st extracellular domain of human CRF1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Sheep, Hamster, Dog, Bovine, Horse, Pig, Opossum (100%), Elephant, Panda, Bat (94%), Marmoset, Mouse, Rat, Seabass, Stickleback, Pufferfish (89%), Turkey, Chicken, Lizard, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Alternative Name: CRFR1 / CRHR1 (CRHR1 Products)

Synonyms: CRF-R antibody, CRF-R-1 antibody, CRF-R1 antibody, CRF1 antibody, CRFR-1 antibody, CRFR1 antibody, CRH-R-1 antibody, CRH-R1 antibody, CRH-R1h antibody, CRHR antibody, CRHR1L antibody, CRHR1f antibody, Crhr antibody, crhr1 antibody, crhr1-A antibody, CRHR1 antibody, CRHR1-IT1-CRHR1 antibody, MGC57346-CRHR1 antibody, corticotropin releasing hormone receptor 1 antibody, corticotropin releasing hormone receptor 1, gene 2 S homeolog antibody, corticotropin-releasing factor receptor 1 antibody, LINC02210-CRHR1 readthrough antibody, CRHR1 antibody, Crhr1 antibody, crhr1.2.S antibody, LOC610380 antibody, LOC100065598 antibody, LINC02210-CRHR1 antibody, crhr1 antibody

Background: Name/Gene ID: CRHR1
Subfamily: Corticotropin releasing factor
Family: GPCR

Synonyms: CRHR1, CRF-R-1, CRFR, CRHR1f, Crf receptor 1, CRH-R1, CRF-R, CRF1, CRFR-1, Crf receptor type 1, Crf receptor type 1, Crf type 1, CRF-R1, Crf1 receptor, CRFR1, CRH-R-1, CRH-R1h, CRHR, CRHR1L

Gene ID: 1394

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity