Insulin-Like Growth Factor 2 MRNA Binding Protein 2 (IGF2BP2) (C-Term) antibody

Details for Product No. ABIN238651
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Antigen
Synonyms IMP-2, IMP2, VICKZ2, p62, C330012H03Rik, Imp2, Neilsen, RGD1305614
Epitope
C-Term
(13), (10), (9), (8), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(81), (21), (17), (13), (10), (7), (1)
Host
Goat
(56), (21), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(65), (31), (23), (20), (18), (10), (10), (7), (3), (3), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN238651
374.27 $
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Immunogen Peptide with sequence C-KQQEQKYPQGVASQR, from the C Terminus of the protein sequence according to NP_006539.3, NP_001007226.1.
Specificity This antibody is expected to recognise both reported isoforms (NP_006539.3 and NP_001007226.1).
Cross-Reactivity Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name IGF2BP2
Background Synonyms: IGF2BP2, insulin-like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, VICKZ2, p62, IGF II mRNA binding protein 2, IGF-II mRNA-binding protein 2
Gene ID 10644, 319765
NCBI Accession NP_006539, NP_001007226
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 60-70 kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1 kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Insulin-Like Growth Factor 2 MRNA Binding Protein 2 (IGF2BP2) (C-Term) antibody ABIN238651 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Zeggini, Weedon, Lindgren et al.: "Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes." in: Science (New York, N.Y.), Vol. 316, Issue 5829, pp. 1336-41, 2007 (PubMed).

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