Neutral Cholesterol Ester Hydrolase 1 (NCEH1) (C-Term) antibody

Details for Product No. ABIN238671
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Antigen
Synonyms AADACL1, nceh, aadacl1, MGC79479, NCEH, Aadacl1, B230106I24Rik, CPO-BP, Nceh, RGD1311104
Epitope
C-Term
(3), (1), (1)
Reactivity
Human, Mouse (Murine), Dog (Canine)
(18), (18), (14), (1)
Host
Goat
(18), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(10), (9), (4), (3)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN238671
261.61 $
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Immunogen Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name AADACL1
Background Synonyms: AADACL1, arylacetamide deacetylase-like 1
Gene ID 57552, 320024
NCBI Accession NP_065843
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Preliminary experiments gave an approx 26-28 kDa band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1 kDa according to NP_065843.3. The 26-28 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Chiang, Niessen, Saghatelian et al.: "An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling." in: Chemistry & biology, Vol. 13, Issue 10, pp. 1041-50, 2006 (PubMed).

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