Forkhead Box P2 (FOXP2) (C-Term) antibody

Antigen: Forkhead Box P2 (FOXP2)

Synonyms: SPCH1, CAGH44, TNRC10, DKFZp686H1726, CAG-16, D0Kist7, AI449000, 2810043D05Rik, FOXP2, RGD1559697, spch1, cagh44, tnrc10, xlFoxP2, foxP2, foxP, Foxp2, foxp1c

Binding Site: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN263146

Additional Information

Alternative name: FOXP2

Gene ID: 93986

Immunogen: C-REIEEEPLSEDLE

Description: All names and symbols: FOXP2, forkhead box P2, SPCH1, CAGH44, TNRC10, CAG repeat protein 44, speech and language disorder 1, trinucleotide repeat containing 10, forkhead/winged-helix transcription factor. Official Symbol: FOXP2. RefSeq number(s): NP_055306.1, NP_683696.2, NP_683697.1. Gene ontology terms: transcription factor activity, neurogenesis, transcription from Pol II promoter

Specificity: This antibody is expected to recognize all three reported isoforms (NP_055306.1, NP_683696.2, NP_683697.1). Human, Mouse, Rat, Dog, Cow, Pig, etc

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 80kDa band observed in human brain (cerebellum) lysates (calculated MW of 79.9kDa according to NP_055306.1). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN263146 (0.5µg/ml) staining of human cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Lai, Fisher, Hurst et al.: "A forkhead-domain gene is mutated in a severe speech and language disorder." in: Nature, Vol. 413, Issue 6855, pp. 519-23, 2001 (PubMed).

Product: Fujita, Sugihara: "FoxP2 expression in the cerebellum and inferior olive: development of the transverse stripe-shaped expression pattern in the mouse cerebellar cortex." in: The Journal of comparative neurology, Vol. 520, Issue 3, pp. 656-77, 2011 (PubMed).