ADAM17 antibody (C-Term)

Catalog No. ABIN264994

Product Details anti-ADAM17 Antibody

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Binding Specificity: AA 807-823, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody Detects TACE (ADAM17/CD156b).

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 807 to 823 of the human TACE protein. Immunogen sequence differs from the mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western Blot (0.25-1 μg/mL): Detects an approximately 130 kDa band in HeLa and Jurkatwhole cell lysate which may represent mature protein, precursor, and glycosylated TACE.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline solution containing 0.02 % Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8 °C.

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: CD156b / ADAM17 (ADAM17 Products)

Synonyms: ADAM18 antibody, CD156B antibody, CSVP antibody, NISBD antibody, TACE antibody, CD156b antibody, Tace antibody, adam17 antibody, si:dkey-81b15.2 antibody, adam18 antibody, cd156b antibody, csvp antibody, tace antibody, fc20b01 antibody, wu:fc20b01 antibody, zgc:63886 antibody, ADAM metallopeptidase domain 17 antibody, a disintegrin and metallopeptidase domain 17 antibody, ADAM metallopeptidase domain 17b antibody, ADAM metallopeptidase domain 17 L homeolog antibody, ADAM metallopeptidase domain 17a antibody, ADAM17 antibody, Adam17 antibody, adam17b antibody, adam17.L antibody, adam17a antibody

Background: Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association. (6)Synonyms: CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme

Gene ID: 6868

NCBI Accession: NP_003174

UniProt: P78536

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus