FASL antibody (PE)

Catalog No. ABIN2663098

Product Details anti-FASL Antibody (PE)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This FASL antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Clone: MFL3

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: CD178 (FASL Products)

Background: CD178 is a 40 kD member of the TNF/NGF superfamily also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The MFL3 antibody recognizes CD178 in a wide array of mouse strains and has been reported to block CD178/CD95 induced apoptosis.

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity