VCP antibody

Catalog No. ABIN284668

Product Details anti-VCP Antibody

Target: VCP (Valosin Containing Protein (VCP))

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB)

Purity: Culture supernatant

Immunogen: p97 ATPase antibody was raised in mouse using 15S Mg2+ ATPase complex purified from xenopus laevis ovary as the immunogen.

Clone: 58-13-3

Isotype: IgG1

Application Details

Application Notes: WB: 1:100-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Concentrated cell culture supernatant with 150 mM PBS, 0.1 % BSA, and 0.1 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Storage: 4 °C

Storage Comment: Store at 4 °C

References for anti-VCP antibody (ABIN284668)

Ge, Tian, Liu, Wong, Gong, Wu, Cho, Lin, Kast, Lu, Wang: "p97 regulates GluA1 homomeric AMPA receptor formation and plasma membrane expression." in: Nature communications, Vol. 10, Issue 1, pp. 4089, (2019) (PubMed).

Zhou, Zhang, Anand, Stomberski, Qian, Hausladen, Wang, Rhee, Parikh, Karumanchi, Stamler: "Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury." in: Nature, Vol. 565, Issue 7737, pp. 96-100, (2019) (PubMed).

Ye, Baek, Ye, Zhang: "Proteomic characterization of endogenous substrates of mammalian ubiquitin ligase Hrd1." in: Cell & bioscience, Vol. 8, pp. 46, (2018) (PubMed).

Fang, Hemion, Pinho Ferreira Bento, Bippes, Flammer, Neutzner: "Mitochondrial function in neuronal cells depends on p97/VCP/Cdc48-mediated quality control." in: Frontiers in cellular neuroscience, Vol. 9, pp. 16, (2015) (PubMed).

Baron, Pedrioli, Tyagi, Johnson, Wood, Fountaine, Wightman, Alexandru: "VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase." in: BMC biology, Vol. 12, pp. 39, (2014) (PubMed).

Jiang, Knox, Pathipati, Ferriero: "Developmental localization of NMDA receptors, Src and MAP kinases in mouse brain." in: Neuroscience letters, Vol. 503, Issue 3, pp. 215-9, (2011) (PubMed).

Ernst, Mueller, Ploegh, Schlieker: "The otubain YOD1 is a deubiquitinating enzyme that associates with p97 to facilitate protein dislocation from the ER." in: Molecular cell, Vol. 36, Issue 1, pp. 28-38, (2009) (PubMed).

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: p97 ATPase (VCP Products)

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway