VCP antibody (C-Term)

Catalog No. ABIN3043958

Product Details anti-VCP Antibody

Target: VCP (Valosin Containing Protein (VCP))

Binding Specificity: AA 732-760, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Transitional endoplasmic reticulum ATPase(VCP) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Sequence: RRDHFEEAMR FARRSVSDND IRKYEMFAQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Transitional endoplasmic reticulum ATPase(VCP) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: valosin containing protein
Protein Name: Transitional endoplasmic reticulum ATPase

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VCP (732-760aa RRDHFEEAMRFARRSVSDNDIRKYEMFAQ), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for VCP is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: VCP (VCP Products)

Background: Valosin-containing protein also called CDC48 is an enzyme that in humans is encoded by the VCP gene. It is a member of the AAA+ (ATPase associated with various activities) protein family. The VCP gene maps to chromosome 9p13.3. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It is involved in the formation of the transitional endoplasmic reticulum. This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. It also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.

Synonyms: 15S Mg(2+) ATPase p97 subunit antibody|15S Mg(2+)-ATPase p97 subunit antibody|ALS14 antibody|ATPase p97 antibody|IBMPFD antibody|MGC131997 antibody|MGC148092 antibody|MGC8560 antibody|p97 antibody|TER ATPase antibody|TERA antibody|TERA_HUMAN antibody|Transitional endoplasmic reticulum ATPase antibody|Valosin containing protein antibody|Valosin-containing protein antibody|VCP antibody|Yeast Cdc48p homolog antibody

Gene ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway