Integrin beta 4 antibody (Extracellular Domain)

Catalog No. ABIN298749

Product Details anti-Integrin beta 4 Antibody

Target: Integrin beta 4 (ITGB4)

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Specificity: Selected for its ability to recognize human Integrin B4 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~25 % cross-reactivity with rmIntegrin B4, and less than 1 % cross-reactivity with rhIntegrin B2, rhIntegrin B3, and rhIntegrin B5.

Purification: Affinity purified

Immunogen: CHO cell-derived rhIntegrin B4 extracellular domain.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Flow Cytometry. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well. Flow cytometry: 50 μg/mL. Add 10 μL to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS with 5 % trehalose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: ITGB4 / Integrin Beta 4 (ITGB4 Products)

Background: Name/Gene ID: ITGB4
Family: Integrin

Synonyms: ITGB4, CD104, Integrin beta-4, Integrin Beta 4, gp150, CD104 antigen, Integrin beta-4 subunit, Integrin, beta 4

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex