CD19 Molecule (CD19) antibody (PerCP)

Antigen: CD19 Molecule (CD19)

Synonyms: B4, MGC12802, AW495831, SPNS1, MGC109570, CD19

Clonality: Monoclonal (LT19)

Host: Mouse

Reactivity: Human

Conjugate: PerCP

Application: Flow Cytometry (FACS)

Catalog no.: ABIN302053

Additional Information

Alternative name: CD19

Immunogen: Daudi human Burkitt lymphoma cell line

Isotype: IgG1

Clone: LT19

Description: CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the timeof heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complexwith CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (Bcell antigen receptor), this complex signals to decrease B cell treshold for activation by theantigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signalindependently of BCR coligation and it turns out to be a central regulatory component uponwhich multiple signaling pathways converge. Mutation of the CD19 gene results inhypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Specificity: The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein(immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it islost on plasma cells.

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.

Publications

Publications: Vantourout, Martinez, Fabre et al.: "Ecto-F1-ATPase and MHC-class I close association on cell membranes." in: Molecular immunology, Vol. 45, Issue 2, pp. 485-92, 2007 (PubMed).

Shao, Suresh, Vakil et al.: "Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1323-33, 2008 (PubMed).

Barat, Gilbert, Imbeault et al.: "Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+ T lymphocytes." in: Retrovirology, Vol. 5, pp. 30, 2008 (PubMed).

Allen, Pang, Skowera et al.: "Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture." in: Diabetes, Vol. 58, Issue 1, pp. 138-45, 2008 (PubMed).

Harrison, Franklin, Campbell: "Enumeration of blood dendritic cells in patients with multiple myeloma at presentation and through therapy." in: Leukemia & lymphoma, Vol. 49, Issue 12, pp. 2272-83, 2008 (PubMed).

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Gonucalves, Castro, Henriques et al.: "Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily." in: Molecular immunology, Vol. 46, Issue 13, pp. 2585-96, 2009 (PubMed).

Rayment, Kooij, Zhang et al.: "Evidence for the specificity for platelet HPA-1a alloepitope and the presenting HLA-DR52a of diverse antigen-specific helper T cell clones from alloimmunized mothers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 677-86, 2009 (PubMed).